X-Gal

ROCHE/XGAL-RO - solubility: in DMF, soluble, solubility: in methanol, soluble, storage temp.:2-8°C

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside; 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside; bromo-4-chloro-3-indolyl-β-d-galactopyranoside, 5-; 5-Bromo-4-chloro-3-indolyl β-D-galactoside; BCIG; X-Gal

CAS Number: 7240-90-6
Empirical Formula (Hill Notation): C₁₄H₁₅BrClNO₆
Molecular Weight: 408.63
MDL Number: MFCD00005666
Linear Formula: C₁₄H₁₅BrClNO₆
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-11680293001	100 mg	$126.00 1/EA	Add To Favorites
45-3117073001	100 mg	$115.00 1/EA	Add To Favorites
45-10651745001	250 mg	$210.00 1/EA	Add To Favorites
45-10745740001	1 g	$530.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

application(s)	genomic analysis life science and biopharma
assay	99%
color	white
concentration	20 mg/mL
description	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside
form	solid
InChI	1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1
InChI key	OPIFSICVWOWJMJ-AEOCFKNESA-N
manufacturer/tradename	Roche
mol wt	408.63 g/mol
packaging	pkg of 1 g (10745740001)
	pkg of 100 mg (03117073001)
	pkg of 100 mg (11680293001)
	pkg of 2.5 g (10703729001)
	pkg of 250 mg (10651745001)
Quality Level	100
SMILES string	OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O
solubility	DMF: soluble
	methanol: soluble
storage temp.	2-8°C
technique(s)	activity assay: suitable

Application:	X-Gal has been used for immunohistocytochemistry applications. It is also the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.
Application:	X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac⁺ genotype.
General description:	X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. It is soluble in dimethylformamide and methanol. The product is not soluble in water.
Other Notes:	For life science research only. Not for use in diagnostic procedures.
Physical form:	Crystals, white powder
Preparation Note:	Working solution: Stock solution:• 20 mg/ml in dimethyl formamide (DMF), clear, colorless solution • A_{590 nm}: 0.01 Storage conditions (working solution): X-gal in solution (i.e., dissolved in DMF 2% (w/v)) is stable for several weeks when stored in the dark at 2 to 8 °C; when the solution is stored at -15 to -25 °C, it is stable for one year.
Quality:	Chromatographically homogeneous

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	99%
Storage Temp.	2-8°C
UNSPSC	12352200

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