X-tremeGENE™ 360 Transfection Reagent
ROCHE/XTG360-RO - Universal polymer reagent for delivering DNA, siRNA, miRNA and CRISPR/RNP to many cell lines
Synonym: transfection reagent
Product Type: Chemical
X-tremeGENE™ 360 Transfection Reagent (0.4 mL)
X-tremeGENE™ 360 Transfection Reagent (5 × 1.0 mL)
Each reagent from the X-tremeGENE™ family is designed to tackle transfection in different cell lines. X-tremeGENE™ 9 is for commonly used cells and X-tremeGENE™ HP is for more difficult to transfect cells. X-tremeGENE™ 360 is a solution for both hard-to-transfect and common cell types. This chart shows performance of each X-tremeGENE™ transfection reagent for various cell lines through mean luminescence (FFLuc RLU).
X-tremeGENE™ 360 Outperforms Lipofectamine® Reagents. A549 (A) or MDCK (B) cells were transfected with luciferase encoding plasmid DNA using either X-tremeGENE™ 360, Lipofectamine® 2000 (Thermo Fisher Scientific) or Lipofectamine® 3000 (Thermo Fisher Scientific) for 24 hours at indicated reagent-to-DNA ratios or reagent-to-P3000™-to-DNA ratio. Transfection was measured by luciferase activity using a conventional assay. Cytotoxicity was assessed by quantifying the LDH released from the cytosol of damaged cells compared to cells alone. Higher transgene expression and lower cytotoxicity was observed in cells transfected with X-tremeGENE™ 360 at optimal ratios compared to cells transfected with Lipofectamine® 2000 or Lipofectamine® 3000.
This image shows HeLa cell transfection using X-tremeGENE™ 360 Transfection Reagent with labeled pDNA (red) and YFP-Nuc expression (green) and membranes are stained with phalloidin. NOTE: Not confocal image, nuclei can be colored yellow. All channels are pseudocolored from a gray-scale camera.
X-tremeGENE™ 360 Outperforms Lipofectamine® for RNP Delivery. Ribonucleoprotein (RNP) complexes composed of PPIB (cyclophilin B) targeting 2-part gRNA (IDT) and Cas9 protein (PNA Bio) were delivered into HEK293T/17 cells using X-tremeGENE™ 360 (1 μL/well) or Lipofectamine® CRISPRMAX™ (1.5 μL/well and 1 μL/well of Lipofectamine® Cas9 Plus™ Reagent, ThermoFisher) or Lipofectamine® RNAiMAX (1.5 μL/well, ThermoFisher) or Lipofectamine® 3000 (1.5 μL/well and 1 μL/well of [PM="P3000"] Reagent, ThermoFisher) in a 24-well format according to the manufacturers’ protocol. Varying levels of gRNA (6 nM or 12 nM) were tested with 6 nM Cas9 protein (PNA Bio). A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.
X-tremeGENE™ 360 Outperforms Lipofectamine® for RNP Delivery. Ribonucleoprotein (RNP) complexes composed of PPIB (cyclophilin B) targeting 2-part gRNA (IDT) and Cas9 protein (PNA Bio) were delivered into U2OS cells using X-tremeGENE™ 360 (1 μL/well) or Lipofectamine® CRISPRMAX™ (1.5 μL/well and 1 μL/well of Lipofectamine® Cas9 Plus™ Reagent, ThermoFisher) or Lipofectamine® RNAiMAX (1.5 μL/well, ThermoFisher) or Lipofectamine® 3000 (1.5 μL/well and 1 μL/well of P3000™ Reagent, ThermoFisher) in a 24-well format according to the manufacturers’ protocol. Varying levels of gRNA (6 nM or 12 nM) were tested with 6 nM Cas9 protein (PNA Bio). A T7E1 mismatch detection assay was used to measure cleavage efficiency at 48 hours post-transfection.
| form | liquid (aqueous solution) |
| grade | for molecular biology |
| manufacturer/tradename | Roche |
| packaging | pkg of 0.4 mL (08724105001) |
| pkg of 1.0 mL (08724121001) | |
| pkg of 5 × 1.0 mL (08724156001) | |
| storage temp. | −20°C |
| technique(s) | transfection: suitable |
| Application: | X-tremeGENE™ 360 Transfection Reagent is a high performance transfection reagent, free of animal-derived components. • Designed to transfect a broad range of eukaryotic cells, many cell lines not transfected well by other reagents, and hard-to-transfect cell lines, such as asK-562 and HepG2. • Can be successfully used in a variety of applications, such as gene expression analysis using transiently transfected cells, generating of stable cell lines, expression of siRNA for gene knockdown studies, and CRISPR gene-editing tools. • Produces minimal cytotoxicity or changes in morphology when adequate numbers of cells are transfected, eliminating the requirement to change media after adding the transfection complex. • Suitable for transient and stable transfection. • Functions well in the presence or absence of serum. |
| Features and Benefits: | X-tremeGENE™ 360 Transfection Reagent is a high-performing, versatile and reliable solution for hard-to-transfect and common cell types. As a proven and reliable reagent, X-tremeGENE™ 360 allows researchers to avoid guesswork when performing transfection. In addition, X-tremeGENE™ 360 exhibits: • Peak performance in a broad range of cell types • Suitable for multiple applications • Efficient delivery of plasmid DNA, siRNA/miRNA and CRISPR/Cas9 components |
| General description: | X-tremeGENE™ 360 Transfection Reagent is an innovative reagent that forms a complex with DNA or RNA and efficiently delivers the nucleic acids into animal or insect cells. With improved utility, X-tremeGENE™ 360 delivers high efficiency transfection in more cell types with low cytotoxicity. |
| Legal Information: | X-tremeGENE is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin. Number of Tests: Using the standard procedure, 1 mL of X-tremeGENE™ 360 Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates. |
| Quality: | Each lot of X-tremeGENE™ 360 Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 360 Transfection Reagent (ratio 3:1 μL/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification. |
| Symbol |   GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 |
| Precautionary statements | P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 - P403 + P235 - P501 |
| WGK Germany | WGK 1 |
| Flash Point(F) | 61.7 °F |
| Flash Point(C) | 16.5 °C |
| Storage Temp. | −20°C |
| UNSPSC | 41106502 |