X-tremeGENE™ 9 DNA Transfection Reagent
ROCHE/XTG9-RO - Polymer reagent for transfecting common cell lines
Synonym: Xtremegene; transfection; transfection reagent
Product Type: Chemical
X-tremeGENE 9 DNA Transfection Reagent
An effective balance of high efficiency and low cytotoxicity. CHO-K1 cells were transfected with a GFP expression plasmid with two different transfection reagents using the reagent/DNA ratio recommended by each of the suppliers. Panels A and C show the fluorescent signals after transfection. Panels B and D are the corresponding bright field photomicrographs. After transfection with LTX P, fluorescent signals are visible, but there are fewer viable cells compared to transfection using X-tremeGENE 9 Transfection Reagent. Cell imaging and analysis was performed using the Roche Cellavista System.
Cytotoxicity comparison with competitor reagents. Reagent cytotoxicity was measured using the Roche LDH Cytotoxicity Detection Kit (LDH), after transfecting HeLa cells with X-tremeGENE 9 DNA Transfection Reagent and two different competitor reagents. The X-tremeGENE 9 DNA Transfection Reagent showed the lowest cytotoxic effect.
Transfection efficiency comparison with reference transfection reagent. Four widely used cell lines were transfected in serum-containing medium using X-tremeGENE 9 Transfection Reagent. Transfection efficiencies were measured as a ratio of GFP-transfected cells versus whole cell number using the Roche Cellavista System, and normalized to the reference transfection reagent set at 1. Error bars show the standard deviation of the mean of triplicates.
High cell survival and reporter gene expression HeLa cells were transfected using X-tremeGENE 9 Transfection Reagent and two different competitor reagents. Reagent cytotoxicity was measured using the Roche LDH Cytotoxicity Detection Kit (LDH). X-tremeGENE 9 DNA Transfection Reagent showed the highest transfection efficiency and the lowest levels of cytotoxicity.
| form | liquid (aqueous solution) |
| grade | for molecular biology |
| manufacturer/tradename | Roche |
| packaging | pkg of 0.4 mL (06365779001) |
| pkg of 1.0 mL (06365787001) | |
| pkg of 5 × 1.0 mL (06365809001) | |
| Quality Level | 100  |
| storage temp. | 2-8°C |
| technique(s) | transfection: suitable |
| usage | mL (suitable for 165 transfections) |
| Application: | X-tremeGENE™ 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis. X-tremeGENE™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as: • Expression of recombinant proteins for functional analysis. • Physiological studies of metabolic pathways. • Analysis of regulatory sequences using reporter gene assays. • Gene expression assays. • Cancer research studies. • Target evaluation. |
| Features and Benefits: | • Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability. • Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum). • Avoid time-consuming optimization procedures in commonly used cell lines. |
| General description: | X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials. |
| Legal Information: | X-tremeGENE is a trademark of Roche |
| Other Notes: | For life science research only. Not for use in diagnostic procedures. |
| Physical form: | Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio. |
| Quality: | Each lot of X-tremeGENE™ 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE™ 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification. |
| Symbol |   GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 |
| Precautionary statements | P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378 |
| RIDADR | UN1170 - class 3 - PG 2 - Ethanol, solution |
| WGK Germany | WGK 1 |
| Flash Point(F) | 334.4 °F |
| Flash Point(C) | 168 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106502 |