N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

SIAL/03450 - purum, ≥98.0% (AT)

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride; EDAC; EDC; EDC hydrochloride; WSC hydrochloride

CAS Number: 25952-53-8
Empirical Formula (Hill Notation): C₈H₁₇N₃ · HCl
Molecular Weight: 191.70
EC Number: 247-361-2
MDL Number: MFCD00012503
Linear Formula: C₈H₁₇N₃ · HCl
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-03450-1G	1 g	$33.80 1/EA	Add To Favorites
45-03450-5G	5 g	$104.00 1/EA	Add To Favorites
45-03450-25G	25 g	$359.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: 03450-1G

Properties
Descriptions
Safety Info.
Basic Data

application(s)	microbiology
assay	≥98.0% (AT)
form	powder
grade	purum
InChI	1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key	FPQQSJJWHUJYPU-UHFFFAOYSA-N
mp	110-115 °C
	110-115 °C (lit.)
Quality Level	200
SMILES string	Cl.CCN=C=NCCCN(C)C
solubility	H₂O: soluble 1 gm/10 ml, clear to very slightly hazy, colorless to very faintly yellow
storage temp.	−20°C

Application:	N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used modify the cell surface of Escherichia coli to covalently couple substances. It has also been used as an activator to modify microfluidic chips to capture Escherichia coli.
Biochem/physiol Actions:	N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide is a water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. Additionally, it reacts with phosphate groups. It has been utilized in peptide synthesis, crosslinking proteins to nucleic acids as well as preparation of immunoconjugates.
Biochem/physiol Actions:	Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.
Features and Benefits:	Versatile and adaptable for a wide variety of laboratory and research applications
General description:	1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC HCl), commonly known as EDAC, is a crucial water-soluble reagent widely employed in chemical and biochemical research, particularly for its proficiency in mediating amide bond formation. Notably effective in peptide synthesis, EDAC HCl demonstrates efficiency in coupling amino acids through their carboxyl and amine groups, enabling the tailored creation of peptides with specific sequences and functionalities. Extending its utility, EDAC HCl plays a pivotal role in the construction of immunogens by covalently attaching haptens—small immune-response eliciting molecules—to carrier proteins, a critical aspect in vaccine research. The adaptability of EDAC HCl encompasses nucleic acid modification, permitting the selective labeling of DNA and RNA through their 5′ phosphate groups. This capacity contributes significantly to the visualization, tracking, and analytical aspects of these fundamental molecules, thereby advancing nucleic acid research. Furthermore, EDAC HCl functions as a biomolecule bridge, acting as a crosslinker connecting amine-reactive NHS-esters of biomolecules to carboxyl groups. This feature proves invaluable in protein conjugation, facilitating the development of hybrid molecules with distinct properties and functions. The underlying reaction mechanism involves EDAC HCl′s interaction with a carboxyl group, forming an unstable intermediate actively seeking an amine partner. The delicate equilibrium of this reaction underscores the necessity for optimizing conditions to ensure efficient conjugation. The assistance of N-hydroxysuccinimide (NHS) enhances the capabilities of EDAC HCl by stabilizing the intermediate and enabling two-step conjugation procedures, affording greater flexibility and control, particularly in the manipulation of complex biomolecular structures.
Other Notes:	For additional information on our range of Biochemicals , please complete this form .

Symbol	GHS06,GHS08,GHS09
Signal word	Danger
Hazard statements	H302 - H311 - H315 - H317 - H373 - H410
Precautionary statements	P260 - P273 - P280 - P301 + P312 - P302 + P352 + P312 - P314
Hazard Codes	Xi
Risk Statements	37/38-41
Safety Statements	26-36/37/39
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥98.0% (AT)
mp	110-115 °C (lit.); 110-115 °C
Storage Temp.	−20°C
UNSPSC	12352111

To Order From a Quote