Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

SIAL/03777 - BioUltra, for molecular biology, ≥99.0% (T)

Synonym: EGTA; Egtazic acid; Ethylene-bis(oxyethylenenitrilo)tetraacetic acid; Glycol ether diamine tetraacetic acid

CAS Number: 67-42-5
Empirical Formula (Hill Notation): C₁₄H₂₄N₂O₁₀
Molecular Weight: 380.35
EC Number: 200-651-2
MDL Number: MFCD00004291
Linear Formula: [-CH₂OCH₂CH₂N(CH₂CO₂H)₂]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-03777-10G	10 g	$201.00 1/EA	Add To Favorites
45-03777-50G	50 g	$604.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

absorption	cut-off at 263 nm in 1 M NaOH at 0.1 M
anion traces	sulfate (SO₄^2-): ≤50 mg/kg
assay	≥99.0% (T)
cation traces	Al: ≤5 mg/kg
	As: ≤0.1 mg/kg
	Ba: ≤5 mg/kg
	Bi: ≤5 mg/kg
	Ca: ≤10 mg/kg
	Cd: ≤5 mg/kg
	Co: ≤5 mg/kg
	Cr: ≤5 mg/kg
	Cu: ≤5 mg/kg
	Fe: ≤5 mg/kg
	K: ≤50 mg/kg
	Li: ≤5 mg/kg
	Mg: ≤5 mg/kg
	Mn: ≤5 mg/kg
	Mo: ≤5 mg/kg
	Na: ≤200 mg/kg
	Ni: ≤5 mg/kg
	Pb: ≤5 mg/kg
	Sr: ≤5 mg/kg
	Zn: ≤5 mg/kg
form	powder
grade	for molecular biology
ign. residue	≤0.05% (as SO₄)
impurities	DNases, none detected
	insoluble matter, passes filter test
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)
InChI key	DEFVIWRASFVYLL-UHFFFAOYSA-N
loss	≤1.0% loss on drying, 110 °C
mp	241 °C (dec.) (lit.)
product line	BioUltra
Quality Level	300
SMILES string	OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O
solubility	0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

Application:	A chelating agent useful for the determination of calcium in the presence of magnesium.
Application:	Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid can be used to study molecular biology, biochemicals, reagents and chelators. Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used in a study that illustrated the response of the β-cyclodextrin-hemin complex in the induction of cucumber adventitious rooting through heme oxygenase-1-dependent mechanism and calcium signaling.
Biochem/physiol Actions:	Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.
Other Notes:	Buffer component in dideoxy sequencing of RNA using reverse transcriptase.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 2
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (T)
mp	241 °C (dec.) (lit.)
UNSPSC	12352200

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