Modified tryptone soy broth
SIAL/08069 - suitable for microbiology, NutriSelect® Plus
Synonym: Modified soybean bile broth base; Tryptic soy broth, modified; mTSB
Product Type: Chemical
Microbial culture media preparation
| agency | according to ISO 16654:2001 |
| application(s) | food and beverages pathogen testing vitamins, nutraceuticals, and natural products |
| microbiology | |
| composition | |
| bile salts mixture, 1.5 g/L | |
| casein enzymic hydrolysate, 17 g/L | |
| dipotassium hydrogen phosphate, 4 g/L | |
| papaic digest of soyabean meal, 3 g/L | |
| sodium chloride, 5 g/L | |
| final pH | 7.3±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective for enterovirulent E. coli (EEC) |
| technique(s) | microbiological culture: suitable |
| Application: | Modified tryptone soy broth is recommended as an enrichment medium for the detection of Escherichia coli O157:H7 from food and animal feeding stuffs. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 33 grams of Modified tryptone soy broth in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121 °C) for 15 minutes. Cool to 45-50 °C and aseptically add rehydrated contents of 1 vial of VCC Selective Supplement (Cat. No. No. 80704). Mix well and dispense as desired. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171607 |