3,3′,5,5′-Tetramethylbenzidine

SIAL/09743 - standard for GC

Synonym: BM blue; Sure Blue TMB; TMB; TMB Blotting Plus; TMB substrate

CAS Number: 54827-17-7
Empirical Formula (Hill Notation): C₁₆H₂₀N₂
Molecular Weight: 240.34
EC Number: 259-364-6
MDL Number: MFCD00007748
Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-09743-250MG	250 mg	$36.90 1/EA	Add To Favorites

Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	environmental
assay	≥99.0% (GC)
format	neat
grade	analytical standard
	standard for GC
InChI	1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key	UAIUNKRWKOVEES-UHFFFAOYSA-N
mp	166-171 °C
	168-171 °C (lit.)
Quality Level	100
shelf life	limited shelf life, expiry date on the label
SMILES string	Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
technique(s)	gas chromatography (GC): suitable
	HPLC: suitable

Application:	3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Application:	3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).
Other Notes:	Standard for determining aromatic amines released from azo-dyes in commodities
Other Notes:	this product will be discontinued, please find the alternative product here : 860336

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (GC)
mp	166-171 °C; 168-171 °C (lit.)
UNSPSC	41116107

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