3,3′,5,5′-Tetramethylbenzidine

SIAL/09743 - standard for GC

Synonym: BM blue; Sure Blue TMB; TMB; TMB Blotting Plus; TMB substrate

CAS Number: 54827-17-7
Empirical Formula (Hill Notation): C₁₆H₂₀N₂
Molecular Weight: 240.34
EC Number: 259-364-6
MDL Number: MFCD00007748
Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-09743-250MG	250 mg	$36.90 1/EA	Add To Favorites

Sigma offers specially formulated TMB liquid substrate systems for ELISA requiring a soluble end-product and for western blotting procedures that require an insoluble end-product. TMB Liquid Substrate System for peroxidase-based ELISA procedures where the soluble blue end product can be read at 370 nm or 655 nm. The addition of an acidic stopping solution produces a yellow color that can be read at 450 nm. The Sigma TMB Substrate System for Membranes produces an insoluble, dark blue end-product, and is not recommended for ELISA or immunohistochemical applications.

Properties
Descriptions
Safety Info.
Basic Data

application(s)	environmental
assay	≥99.0% (GC)
format	neat
grade	analytical standard
	standard for GC
InChI	1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key	UAIUNKRWKOVEES-UHFFFAOYSA-N
mp	166-171 °C
	168-171 °C (lit.)
Quality Level	100
shelf life	limited shelf life, expiry date on the label
SMILES string	Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
technique(s)	gas chromatography (GC): suitable
	HPLC: suitable

Application:	3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.
Application:	3,3′,5,5′-Tetramethylbenzidine may be used as a substrate for the analysis of diclofenac in water samples and dextromethorphan and its major metabolite dextrorphan in urine samples using enzyme-linked immunosorbent assay (ELISA) and gas chromatography coupled to mass spectrometry (GC-MS).
Other Notes:	Standard for determining aromatic amines released from azo-dyes in commodities
Other Notes:	this product will be discontinued, please find the alternative product here : 860336
Recommended products:	Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial .

Hazard Codes	Xi
Risk Statements	36/37/38
Safety Statements	26-36
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.0% (GC)
mp	166-171 °C; 168-171 °C (lit.)
UNSPSC	41116107

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