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Bromocresol Purple

SIAL/114375 - BioReagent, suitable for indicator, Dye content 90 %

Synonym: 5,5′-Dibromo-o-cresolsulfonphthalein; Bromcresol purple sultone form

CAS Number: 115-40-2
Empirical Formula (Hill Notation): C21H16Br2O5S
Molecular Weight: 540.22
EC Number: 204-087-8
MDL Number: MFCD00011681
Linear Formula: C21H16Br2O5S
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-114375-5G 5 g
$71.40
1/EA
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45-114375-25G 25 g
$212.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114375-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114375-5G

 

ε (extinction coefficient) ≥22000 at 418-424 nm in methanol
λmax 419 nm
application(s) diagnostic assay manufacturing
hematology
histology
assay ≥89.0% (HPLC)
composition Dye content, 90%
form powder
InChI 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
InChI key ABIUHPWEYMSGSR-UHFFFAOYSA-N
mp 240 °C (dec.) (lit.)
pH 5.2-6.8, yellow to purple
product line BioReagent
Quality Level 200 
SMILES string Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
solubility ethanol: 1 mg/mL
storage temp. room temp
suitability suitable for indicator
technique(s) titration: suitable
visual transition interval 5.2-6.8, yellow to blue
Application: Bromocresol Purple has been used for staining of Saccharomyces cerevisiae.
Application: Spectrophotometric determination of serum-albumin
Biochem/physiol Actions: Bromocresol purple is a pH indicator which changes color from yellow (at low pH 5.2) to violet (above pH 6.8). It is mainly used as a fluorescent stain in yeast cells. It helps in dead cell count for cells with plasma membrane damage. Cells with proper cell membrane are not stained, while damaged cells are seen as blue-grey ghosts.
Packaging: 5, 25 g in glass bottle
Hazard Codes Xi
Risk Statements 36/37/38
Safety Statements 26-36
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Purity ≥89.0% (HPLC)
mp 240 °C (dec.) (lit.)
Storage Temp. room temp
UNSPSC 12171500

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