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Bromophenol Blue

SIAL/114391 - ACS reagent

Synonym: 3′,3″,5′,5″-Tetrabromophenolsulfonphthalein; Bromphenol Blue Sultone Form

CAS Number: 115-39-9
Empirical Formula (Hill Notation): C19H10Br4O5S
Molecular Weight: 669.96
EC Number: 204-086-2
MDL Number: MFCD00005875
Linear Formula: C19H10Br4O5S
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-114391-5G 5 g
 $32.10
1/EA		 Add To Favorites
45-114391-25G 25 g
 $103.00
1/EA		 Add To Favorites
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114391-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114391-5G

 

application(s) diagnostic assay manufacturing
hematology
histology
form powder
grade ACS reagent
InChI 1S/C19H10Br4O5S/c20-12-5-9(6-13(21)17(12)24)19(10-7-14(22)18(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28-19/h1-8,24-25H
InChI key UDSAIICHUKSCKT-UHFFFAOYSA-N
mp 273 °C (lit.)
Quality Level 200 
SMILES string Oc1c(Br)cc(cc1Br)C2(OS(=O)(=O)c3ccccc23)c4cc(Br)c(O)c(Br)c4
solubility ethanol: 0.1%
storage temp. room temp
technique(s) titration: suitable
visual transition interval 3.0-4.6, yellow to blue
Application: Bromphenol Blue is used as a tracking dye in DNA, RNA (agarose), and protein (polyacrylamide) gel electrophoresis. It has been used in the preparation of protein samples for western blotting analysis. Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. It has also been known for its use as a vital stain to probe the blood-brain barrier.
General description: Bromophenol blue is present as a sulfonated hydroxyquinone in acidic aqueous solutions. It is also known as tetrabromophenolsulfonphthalein.Bromophenol Blue is a pH indicator and a dye appearing as a strong blue color. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.
Packaging: 5, 25 g in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp 273 °C (lit.)
Storage Temp. room temp
UNSPSC 12171500

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