Bromothymol Blue
SIAL/114413 - ACS reagent, Dye content 95 %
Synonym: 3′,3″-Dibromothymolsulfonphthalein
CAS Number: 76-59-5
Empirical Formula (Hill Notation): C27H28Br2O5S
Molecular Weight: 624.38
EC Number: 200-971-2
MDL Number: MFCD00005872
Linear Formula: C27H28Br2O5S
Product Type: Chemical
ε (extinction coefficient) | ≥17000 at 419-425 nm in methanol |
≥8000 at 326-332 nm in methanol | |
≥9000 at 279-285 nm in methanol | |
λmax | 420 nm |
color | pink-purple |
composition | Dye content, 95% |
form | powder |
grade | ACS reagent |
InChI | 1S/C27H28Br2O5S/c1-13(2)1 |
InChI key | NUHCTOLBWMJMLX-UHFFFAOYSA |
mp | 200-202 °C ((392 - 396 °F) - lit.) |
200-202 °C (lit.) | |
Quality Level | 200 |
SMILES string | CC(C)c1cc(c(C)c(Br)c1O)C2 |
storage temp. | room temp |
visual transition interval | 6.0-7.6, yellow to blue(passes test) |
Application: | Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include: • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope • for the demonstration of fungal hyphae within plant roots • in the assessment of the soluble acidity within single rice grains • use in an agar gel medium for enumeration of Bacillus cereus • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar. |
General description: | Bromothymol Blue, also known as, dibromothymolsulfonphthal |
Packaging: | 5, 25 g in glass bottle |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
mp | 200-202 °C ((392 - 396 °F) - lit.); 200-202 °C (lit.) |
Storage Temp. | room temp |
UNSPSC | 12171500 |