Bromothymol Blue
SIAL/114413 - ACS reagent, Dye content 95 %
Synonym: 3′,3″-Dibromothymolsulfonphthalein
CAS Number: 76-59-5
Empirical Formula (Hill Notation): C27H28Br2O5S
Molecular Weight: 624.38
EC Number: 200-971-2
MDL Number: MFCD00005872
Linear Formula: C27H28Br2O5S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114413-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114413-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114413-5G
| ε (extinction coefficient) | ≥17000 at 419-425 nm in methanol |
| ≥8000 at 326-332 nm in methanol | |
| ≥9000 at 279-285 nm in methanol | |
| λmax | 420 nm |
| composition | Dye content, 95% |
| form | powder |
| grade | ACS reagent |
| InChI | 1S/C27H28Br2O5S/c1-13(2)1 |
| InChI key | NUHCTOLBWMJMLX-UHFFFAOYSA |
| mp | 200-202 °C (lit.) |
| Quality Level | 200  |
| SMILES string | CC(C)c1cc(c(C)c(Br)c1O)C2 |
| visual transition interval | 6.0-7.6, yellow to blue(passes test) |
| Application: | Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include: • as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope • for the demonstration of fungal hyphae within plant roots • in the assessment of the soluble acidity within single rice grains • use in an agar gel medium for enumeration of Bacillus cereus • as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar. |
| General description: | Bromothymol Blue is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthal |
| Packaging: | 5, 25 g in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 200-202 °C (lit.) |
| UNSPSC | 12171500 |