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Bromothymol Blue

SIAL/114413 - ACS reagent, Dye content 95 %

Synonym: 3′,3″-Dibromothymolsulfonphthalein

CAS Number: 76-59-5
Empirical Formula (Hill Notation): C27H28Br2O5S
Molecular Weight: 624.38
EC Number: 200-971-2
MDL Number: MFCD00005872
Linear Formula: C27H28Br2O5S
Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-114413-5G 5 g
$36.80
1/EA
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45-114413-25G 25 g
$127.00
1/EA
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114413-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114413-5G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 114413-5G

 

ε (extinction coefficient) ≥17000 at 419-425 nm in methanol
  ≥8000 at 326-332 nm in methanol
  ≥9000 at 279-285 nm in methanol
λmax 420 nm
color pink-purple
composition Dye content, 95%
form powder
grade ACS reagent
InChI 1S/C27H28Br2O5S/c1-13(2)17-11-20(15(5)23(28)25(17)30)27(19-9-7-8-10-22(19)35(32,33)34-27)21-12-18(14(3)4)26(31)24(29)16(21)6/h7-14,30-31H,1-6H3
InChI key NUHCTOLBWMJMLX-UHFFFAOYSA-N
mp 200-202 °C ((392 - 396 °F) - lit.)
  200-202 °C (lit.)
Quality Level 200 
SMILES string CC(C)c1cc(c(C)c(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)c(Br)c4C
storage temp. room temp
visual transition interval 6.0-7.6, yellow to blue(passes test)
Application: Bromothymol blue dye serves as a pH indicator because of the precise color transition at neutrality. Other applications include: •  as a vital stain to trace the movement of fluids from the lymph and to define cell walls or nuclei under the microscope
•  for the demonstration of fungal hyphae within plant roots
• in the assessment of the soluble acidity within single rice grains
• use in an agar gel medium for enumeration of Bacillus cereus
• as a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.
General description: Bromothymol Blue, also known as, dibromothymolsulfonphthalein is a sulfonated hydroxyquinone in acidic aqueous solutions. In alkaline solutions, this dye is present as a moderately sized dianion. It is also known as bromthymol blue and dibromothymolsulfonphthalein. It is a pH-sensitive dye that changes color from yellow to blue.
Packaging: 5, 25 g in glass bottle
RIDADR NONH for all modes of transport
WGK Germany WGK 3
Flash Point(F) Not applicable
Flash Point(C) Not applicable
mp 200-202 °C ((392 - 396 °F) - lit.); 200-202 °C (lit.)
Storage Temp. room temp
UNSPSC 12171500

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