Indigo carmine
SIAL/131164 - certified by the Biological Stain Commission, Dye content 85 %
Synonym: Acid Blue 74; Indigo-5,5′-disulfonic acid disodium salt; Indigocarmine
CAS Number: 860-22-0
Empirical Formula (Hill Notation): C16H8N2Na2O8S2
Molecular Weight: 466.35
EC Number: 212-728-8
MDL Number: MFCD00005723
Linear Formula: C16H8N2Na2O8S2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 131164-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 131164-25G
| εmax | 20,000 at 250-256 nm in water at 0.01 g/L |
| 29500 at 284-290 nm in water at 0.01 g/L | |
| λmax | 608 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | >85.0% (HPLC) |
| color | dark blue to very dark blue |
| composition | Dye content, 85% |
| density | 0.71 g/cm3 at 29 °C |
| form | solid |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C16H10N2O8S2.2Na/c19-1 |
| InChI key | KHLVKKOJDHCJMG-QDBORUFSSA |
| mp | ≥340 °C |
| pH | 3.87 (30 °C, 7.14 g/L) |
| Quality Level | 200  |
| SMILES string | [Na+].[Na+].[O-]S(=O)(=O) |
| storage temp. | room temp |
| Application: | Indigo carmine has been used to investigate its effects on ozone production by neutrophils. |
| Biochem/physiol Actions: | Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method. |
| Packaging: | 25, 100 g in glass bottle |
| Suitability: | Used as a collagen stain and in procedures using red nuclear stains. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| Safety Statements | 22-24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Purity | >85.0% (HPLC) |
| mp | ≥340 °C |
| Density | 0.71 g/cm3 at 29 °C |
| Storage Temp. | room temp |
| Colour Index Number | 73015 |
| UNSPSC | 12171500 |