Acridine Orange hemi(zinc chloride) salt
SIAL/158550 - Dye content 85 %
Synonym: 3,6-
CAS Number: 10127-02-3
Empirical Formula (Hill Notation): C17H19N3 · HCl · 0.5ZnCl2
Molecular Weight: 369.96
EC Number: 233-353-6
MDL Number: MFCD00081043
Linear Formula: C17H20ClN3 · HCl · 1/2ZnCl2
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 158550-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 158550-10G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 158550-25G
| λmax | 489 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| composition | Dye content, 85% |
| form | powder |
| InChI | 1S/2C17H19N3.4ClH.Zn/c2*1 |
| InChI key | RAHGLSRJKRXOSY-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | Cl[H].Cl[H].Cl[Zn]Cl.CN(C |
| solubility | H2O: 1 mg/mL, clear, orange to very deep orange |
| storage temp. | room temp |
| Application: | Acridine Orange hemi (zinc chloride) salt has been used to stain primary human umbilical vein endothelial cells (HUVEC) and is used in micronucleus tests (MNTs). |
| Application: | Acridine Orange, a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA, is used in fluorescence and epiflouresence microscopy. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry, characterize multidrug resistance, and measure changes in mitochondrial mass during apoptosis in rat thymocytes. |
| Biochem/physiol Actions: | Acridine orange (AO) is a fluorescent dye with two different staining characteristics. In fixed tissues, it acts as a metachromatic dye and differentially stains DNA (green) and RNA (orange). But, in living cells it acts as a pH indicator and produce brilliant orange-red in acidic condition. AO has been used to identify apoptotic cells in drosophila embryos. Acridine Orange staining method has been used to deliver more consistent data in the micronucleus test. |
| Packaging: | 10, 25, 100 g in glass bottle |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H341 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 68 |
| Safety Statements | 36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 46005 |
| UNSPSC | 12171500 |