AEA Sporulation Broth (Base), modified
SIAL/17170 - suitable for microbiology, NutriSelect® Plus
Product Type: Chemical
| application(s) | food and beverages |
| microbiology | |
| composition | ammonium acetate, 1.5 g/L |
| biopeptone, 10 g/L | |
| disodium phosphate, 4.36 g/L | |
| magnesium sulfate.7H2O, 0.2 g/L | |
| monopotassium phosphate, 0.25 g/L | |
| yeast extract, 10 g/L | |
| final pH | 7.8±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | nonselective for Clostridium spp. |
| technique(s) | microbiological culture: suitable |
| Application: | For early sporulation of Clostridium perfringens from foods. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 26.31 g in 1 litre distilled water. Dispense the medium in 15 ml amounts in screw capped tubes and sterilize by autoclaving at 121°C for 15 minutes. Cool to 45-50°C and add 0.6 ml of filter sterilized 10% raffinose and 0.2 ml each of sterile 0.66 M sodium carbonate and 0.32% cobalt chloride dropwise to each 15 ml base medium in the tubes. Just before use, steam the medium for 10 minutes and after cooling, add 0.2 ml of filter sterilized (freshly prepared) 1.5% sodium ascorbate to each tube of the medium. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |