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2,3,5-Triphenyl-tetrazolium chloride solution

SIAL/17779 - suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Synonym: TTC solution, TTC supplement; TTC solution; TTC supplement

CAS Number: 298-96-4
Empirical Formula (Hill Notation): C19H15ClN4
Molecular Weight: 334.80
MDL Number: MFCD00011963
Linear Formula: C19H15ClN4
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-17779-10X10ML-F 10 amp
 $281.00
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application(s) agriculture
environmental
food and beverages
  microbiology
form liquid
InChI 1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1
InChI key PKDBCJSWQUOKDO-UHFFFAOYSA-M
mol wt 334.8 g/mol
Quality Level 100 
shelf life limited shelf life, expiry date on the label
SMILES string [Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4
sterility sterile (Filtered and Aseptic Handled)
storage temp. 2-8°C
suitability bacteria
Application: 2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media
General description: 2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.
Other Notes: (per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml
RIDADR NONH for all modes of transport
WGK Germany WGK 2
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. 2-8°C
UNSPSC 41171614

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