Ninhydrin reagent according to Stahl
SIAL/17975 - for TLC derivatization
Product Type: Chemical
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| density | 0.823 g/mL at 20 °C |
| form | liquid |
| impurities | 1-butanol |
| acetic acid | |
| Quality Level | 100  |
| technique(s) | thin layer chromatography (TLC): suitable |
| Application: | 1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid |
| Application: | Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates. |
| General description: | Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system. |
| Symbol |    GHS02,GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H226 - H302 - H315 - H318 - H335 - H336 |
| Precautionary statements | P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-37/38-41-67 |
| Safety Statements | 26-39 |
| RIDADR | UN1120 - class 3 - PG 3 - Butanols |
| WGK Germany | WGK 2 |
| Flash Point(F) | 93.2 °F - closed cup |
| Flash Point(C) | 34 °C - closed cup |
| Density | 0.823 g/mL at 20 °C |
| UNSPSC | 41116105 |