Brucella Agar
SIAL/18795 - suitable for microbiology, NutriSelect® Basic
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 18795-500G
| application(s) | cleaning products clinical testing cosmetics environmental food and beverages personal care veterinary |
| microbiology | |
| composition | |
| agar, 13 g/L | |
| casein peptone, 10 g/L | |
| meat peptone, 10 g/L | |
| sodium chloride, 5 g/L | |
| yeast extract, 2 g/L | |
| final pH | 7.0±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | Haemophilus spp. |
| Pneumococcus spp. | |
| nonselective for Brucella spp. | |
| nonselective for Listeria spp. | |
| nonselective for Neisseria spp. | |
| nonselective for Streptococcus spp. | |
| technique(s) | microbiological culture: suitable |
| Application: | For the isolation and cultivation of Brucella species, in particular the pathogens B. melitensis, B. abortus and B. suis, from clinical material and comestibles of animal origin. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Dissolve 41 g in 1 litre distilled water and autoclave at 121°C for 15 minutes. Let cool to 45-50°C and add the supplement according the data sheet. Supplements : Brucella differential agar: 1 ml/l 2% basic fuchsin solution (Cat. No. 03968). or 2% solution from thionine acetate (Cat. No. 88930) Brucella selective agar: 500 mg/l Bacitracin (Cat. No. 11702) 1 mg/l Polymyxin B sulfate (Cat. No. P4932) 100 mg/l Cycloheximide (Cat. No. 01810) (and if required) 1.25 mg/l ethyl violet (Cat. No. 228842) For isolation of Campylobacter species: 50 ml/l heat inactivated horse serum (Cat. No. H1138) 250mg/l Sodium pyruvate (Cat. No. P2256) 250mg/l Sodium metabisulfite (Cat. No. 31448) 250mg/l Ferrous sulfate (Cat. No. 31236) 50-100 ml/l defibrinated sheep blood |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171607 |