Toluidine Blue O
SIAL/198161 - certified by the Biological Stain Commission
Synonym: Basic Blue 17; Blutene chloride; Methylene Blue T50 or T extra; Tolonium chloride
CAS Number: 92-31-9
Empirical Formula (Hill Notation): C15H16ClN3S
Molecular Weight: 305.83
EC Number: 202-146-2
MDL Number: MFCD00011934
Linear Formula: C15H16ClN3S
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 198161-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 198161-5G
| ε (extinction coefficient) | ≥13000 at 241-247 nm in H2O |
| ≥28000 at 287-293 nm in H2O | |
| ≥40000 at 625-635 nm in H2O | |
| λmax | 626 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark green |
| composition | Dye content, 80% |
| form | powder |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C15H15N3S.ClH/c1-9-6-1 |
| InChI key | GEDVVYWLPUPJJZ-UHFFFAOYSA |
| Quality Level | 200  |
| SMILES string | [Cl-].Cc1cc2N=C3C=CC(C=C |
| storage temp. | room temp |
| Application: | Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria. |
| Biochem/physiol Actions: | Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin. |
| General description: | A metachromatic dye suitable for a wide variety of histological staining procedures. |
| Packaging: | 5, 25 g in glass bottle |
| Suitability: | Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | room temp |
| Colour Index Number | 52040 |
| UNSPSC | 12171500 |