Sudan Black B
SIAL/199664 - certified by the Biological Stain Commission
Synonym: Ceres black BN; Fat Black HB; Solvent Black 3
CAS Number: 4197-25-5
Empirical Formula (Hill Notation): C29H24N6
Molecular Weight: 456.54
EC Number: 224-087-1
MDL Number: MFCD00006919
Linear Formula: C29H24N6
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 199664-25G
| ε (extinction coefficient) | ≥20000 at 596-603 nm in ethanol |
| λmax | 415 nm (2nd) |
| 598 nm | |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark brown to very dark brown |
| form | powder |
| grade | certified by the Biological Stain Commission |
| InChI | 1S/C29H24N6/c1-29(2)30-26 |
| InChI key | YCUVUDODLRLVIC-VPHDGDOJSA |
| mp | 120-124 °C (lit.) |
| Quality Level | 200  |
| SMILES string | CC1(C)Nc2cccc3c(ccc(N1)c2 |
| storage temp. | room temp |
| Application: | Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection. |
| Biochem/physiol Actions: | Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn’t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black. |
| Packaging: | 25 g in glass bottle |
| Suitability: | Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid. |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H341 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| mp | 120-124 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 26150; 26150 |
| UNSPSC | 12171500 |