Formaldehyde solution
SIAL/252549 - ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)
Synonym: Formalin
CAS Number: 50-00-0
Empirical Formula (Hill Notation): CH2O
Molecular Weight: 30.03
MDL Number: MFCD00003274
Linear Formula: HCHO
Product Type: Chemical
| anion traces | chloride (Cl-): ≤5 ppm |
| sulfate (SO42-): ≤0.002% | |
| autoignition temp. | 572 °F |
| cation traces | Fe: ≤5 ppm |
| heavy metals: ≤5 ppm (by ICP-OES) | |
| color | APHA: ≤10 |
| concentration | 36.5-38.0% |
| 37 wt. % in H2O | |
| contains | 10-15% Methanol as stabilizer (to prevent polymerization) |
| density | 1.09 g/mL at 25 °C (lit.) |
| form | liquid |
| grade | ACS reagent |
| ign. residue | ≤0.005% |
| impurities | ≤0.006 meq/g Titr. acid |
| InChI | 1S/CH2O/c1-2/h1H2 |
| InChI key | WSFSSNUMVMOOMR-UHFFFAOYSA |
| Quality Level | 200 ![]() |
| refractive index | n |
| SMILES string | [H]C([H])=O |
| solubility | water: soluble |
| suitability | suitable for manufacturing use (Laboratory chemicals) |
| vapor density | 1.03 (vs air) |
| vapor pressure | 52 mmHg ( 37 °C) |
| Application: | Formaldehyde solution has been used as a fixing agent to fix cells during immunofluorescence imaging and for cross-linking cells during chromatin immunoprecipitation (ChIP) assay. |
| Application: | Formaldehyde solution has been used for cross-linking/fixing of cells in ChIP (chromatin immunoprecipitation) assay. It has been used for fixing of cells for imaging. |
| General description: | Formaldehyde (formalin) is produced by oxidation of methanol. It is made of 37% formaldehyde and impurities such as methanol, small amounts of formic acid, aldehydes and ketones. It is used as a denaturant in formaldehyde-agarose gel electrophoresis of RNA. |
| Packaging: | 1, 4 L in glass bottle |
| Packaging: | 25, 100, 4×100, 500, 6×500 mL in glass bottle |
| Physical form: | This product is a solution of approximately 37% by weight of formaldehyde gas in water. |
| Symbol | ![]() ![]() ![]() GHS02,GHS05,GHS06,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H301 + H311 - H314 - H317 - H330 - H335 - H341 - H350 - H370 |
| Precautionary statements | P201 - P210 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 23/24/25-34-39/23/24/25-40-43 |
| Safety Statements | 26-36/37/39-45-51 |
| RIDADR | UN 2209 8 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | 132.8 °F - closed cup |
| Flash Point(C) | 56 °C - closed cup |
| Density | 1.09 g/mL at 25 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12352114 |





