Tris(hydroxymethyl)aminomethane

SIAL/252859 - ACS reagent, ≥99.8%

Synonym: Tris(hydroxymethyl)aminomethane; 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Trometamol

CAS Number: 77-86-1
Empirical Formula (Hill Notation): C₄H₁₁NO₃
Molecular Weight: 121.14
EC Number: 201-064-4
MDL Number: MFCD00004679
Linear Formula: NH₂C(CH₂OH)₃
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-252859-100G	100 g	$56.30 1/EA	Add To Favorites
45-252859-500G	500 g	$138.00 1/EA	Add To Favorites

This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material.

This image depicts SKU: 252859-100G

Properties
Descriptions
Safety Info.
Basic Data

absorption	passes test
application(s)	clinical research diagnostic assay manufacturing microbiology
assay	≥99.8%
	99.8-100.1%
bp	219-220 °C/10 mmHg (lit.)
cation traces	Fe: ≤5 ppm
	heavy metals: ≤5 ppm (ICP)
color	white
foreign activity	DNase, none detected
	NICKase, none detected
	protease, none detected
	RNase, none detected
form	crystalline
grade	ACS reagent
impurities	≤0.005% insolubles
	≤2% water
InChI	1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key	LENZDBCJOHFCAS-UHFFFAOYSA-N
mp	167-172 °C (lit.)
pKa (25 °C)	8.1
Quality Level	200
SMILES string	NC(CO)(CO)CO
technique(s)	affinity chromatography: suitable
useful pH range	7-9

Application:	Tris(hydroxymethyl)aminomethane has been used:• as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells. • as a buffer in flow cell sample preparation for Raman spectroscopy • as a wash buffer in Western blotting • as a buffer component in decellularization of tissues
Features and Benefits:	• Efficient buffering within the pH range of 7-9 with a pKa of 8.1 (25 °C) • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Can be used in Cell Biology, and Biochemical research
General description:	Tris(hydroxymethyl)aminomethane, commonly known as Trometamol, Tris base, or Trizma base, plays a crucial role in diverse research applications as a biological buffer. It is extensively used in the formulation of buffers like Tris-acetate-EDTA (TAE) and Tris-borate-EDTA (TBE), owing to its optimal pKa of 8.1, making it well-suited for maintaining pH within the physiological range (pH 7 -9) applicable to most living organisms. However, caution might be necessary when studying some proteins, as Tris has the potential to interfere with the activity of certain enzymes. Tris base can be employed both independently as a buffer and as a component in mixed buffer formulations, including Tris-EDTA (TE) buffer, TAE buffer, and TBE buffer, among others. Importantly, Tris base is characterized by its purity, essential stability, and relative non-hygroscopic nature. In laboratory settings, Tris base is indispensable for the preparation of buffers compatible with biological fluids, acting as a standard pH solution. It facilitates various procedures such as lactate dehydrogenase assays, in situ hybridization, and protein extraction from cells. Its versatility extends to applications in cell culture, biochemistry, and molecular biology labs, where it contributes to studies involving cell membrane permeability and buffer preparation.
Legal Information:	Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes:	For additional information on our range of Biochemicals , please complete this form .
Packaging:	100, 500 g in poly bottle

RIDADR	NONH for all modes of transport
WGK Germany	WGK 1
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99.8%; 99.8-100.1%
bp	219-220 °C/10 mmHg (lit.)
mp	167-172 °C (lit.)
UNSPSC	12352104

To Order From a Quote