Chloroform – isoamyl alcohol mixture
SIAL/25668 - BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)
Synonym: Chloroform:Isoamyl Alcohol Solvent Mix
MDL Number: MFCD00171265
Product Type: Chemical
λ | neat |
assay | ≥99.5% (chloroform + isoamyl alcohol, GC) |
cation traces | Al: ≤0.5 mg/kg |
Ba: ≤0.1 mg/kg | |
Bi: ≤0.1 mg/kg | |
Ca: ≤0.5 mg/kg | |
Cd: ≤0.05 mg/kg | |
Co: ≤0.02 mg/kg | |
Cr: ≤0.02 mg/kg | |
Cu: ≤0.02 mg/kg | |
Fe: ≤0.1 mg/kg | |
K: ≤0.5 mg/kg | |
Li: ≤0.1 mg/kg | |
Mg: ≤0.1 mg/kg | |
Mn: ≤0.02 mg/kg | |
Mo: ≤0.1 mg/kg | |
Na: ≤0.5 mg/kg | |
Ni: ≤0.02 mg/kg | |
Pb: ≤0.1 mg/kg | |
Sr: ≤0.1 mg/kg | |
Zn: ≤0.1 mg/kg | |
form | liquid |
grade | for molecular biology |
impurities | DNases, none detected |
insoluble matter, passes filter test | |
phosphatases, none detected | |
proteases, none detected | |
RNases, none detected | |
≤0.001% free alkali (as NH3) | |
InChI | 1S/C5H12O.CHCl3/c1-5(2)3- |
InChI key | BKHZIBWEHPHYAI-UHFFFAOYSA |
product line | BioUltra |
Quality Level | 200 |
SMILES string | ClC(Cl)Cl.CC(C)CCO |
UV absorption | λ: 260 nm Amax: 0.1 |
λ: 280 nm Amax: 0.07 |
Application: | Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions. |
Components: | mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v) |
Symbol | GHS06,GHS08 |
Signal word | Danger |
Hazard statements | H302 - H315 - H319 - H331 - H336 - H351 - H361d - H372 - H412 |
Precautionary statements | P201 - P273 - P301 + P312 + P330 - P302 + P352 - P304 + P340 + P311 - P308 + P313 |
Hazard Codes | Xn |
Risk Statements | 63-20/22-36/38-40-48/20/22 |
Safety Statements | 26-36/37 |
RIDADR | UN 1888 6.1 / PGIII |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Purity | ≥99.5% (chloroform + isoamyl alcohol, GC) |
UNSPSC | 12352401 |