Chloroform – isoamyl alcohol mixture

SIAL/25668 - BioUltra, Molecular Biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Synonym: Chloroform:Isoamyl Alcohol Solvent Mix

MDL Number: MFCD00171265
Product Type: Chemical

λ	neat
assay	≥99.5% (chloroform + isoamyl alcohol, GC)
cation traces	Al: ≤0.5 mg/kg
	Ba: ≤0.1 mg/kg
	Bi: ≤0.1 mg/kg
	Ca: ≤0.5 mg/kg
	Cd: ≤0.05 mg/kg
	Co: ≤0.02 mg/kg
	Cr: ≤0.02 mg/kg
	Cu: ≤0.02 mg/kg
	Fe: ≤0.1 mg/kg
	K: ≤0.5 mg/kg
	Li: ≤0.1 mg/kg
	Mg: ≤0.1 mg/kg
	Mn: ≤0.02 mg/kg
	Mo: ≤0.1 mg/kg
	Na: ≤0.5 mg/kg
	Ni: ≤0.02 mg/kg
	Pb: ≤0.1 mg/kg
	Sr: ≤0.1 mg/kg
	Zn: ≤0.1 mg/kg
form	liquid
grade	Molecular Biology
impurities	DNases, none detected
	insoluble matter, passes filter test
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
	≤0.001% free alkali (as NH₃)
InChI	1S/C5H12O.CHCl3/c1-5(2)3-4-6;2-1(3)4/h5-6H,3-4H2,1-2H3;1H
InChI key	BKHZIBWEHPHYAI-UHFFFAOYSA-N
product line	BioUltra
Quality Level	200
SMILES string	ClC(Cl)Cl.CC(C)CCO
technique(s)	GC/GC: suitable
UV absorption	λ: 260 nm A_max: 0.1
	λ: 280 nm A_max: 0.07

Application:	Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.
Other Notes:	mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)

Purity	≥99.5% (chloroform + isoamyl alcohol, GC)
UNSPSC	12352401