Methylene Blue hydrate
SIAL/28514 - ≥95% (calc. to the dried substance)
CAS Number: 122965-43-9
Empirical Formula (Hill Notation): C16H18ClN3S · xH2O
Molecular Weight: 319.85 (anhydrous basis)
EC Number: 200-515-2
MDL Number: MFCD00150006
Linear Formula: C16H18ClN3S · xH2O
Product Type: Chemical
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥95% (calc. to the dried substance) |
| form | powder |
| impurities | ≤1% insoluble in ethanol |
| InChI | 1S/C16H18N3S.ClH.H2O/c1-1 |
| InChI key | WQVSELLRAGBDLX-UHFFFAOYSA |
| loss | ≤22% loss on drying, 105 °C |
| Quality Level | 200  |
| SMILES string | O.[Cl-].CN(C)c1ccc2N=C3C= |
| solubility | ethanol: insoluble ≤1% |
| storage temp. | room temp |
| Application: | Methylene blue is widely used in several routine staining applications including Mallory’s phloxine-methylene blue on paraffin sections of tissue fixed in Zenker’s fluid for nuclear staining and bacterial staining in milk. It is also suitable for manufacturing the polychrome methylene blue used in the preparation of Romanowsky stains.Methylene Blue hydrate has also been used:• in the preparation of methylene blue (MB) solution • to stain zymograms -to label the injection site in the tissue slices • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss |
| General description: | Methylene blue is bis(dimethylamino)phenoth C16H18N3SCl. It is a major basic histological dye in pharmacology and microbiology. This heterocyclic aromatic chemical appears as a dark green solid powder, changing to blue in an aqueous solution. Methylene blue is tested for histological and and bacteriological staining, and as a constituent of Wright’s blood stain. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Precautionary statements | P264 - P270 - P301 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (calc. to the dried substance) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |