Fluorescein sodium salt
SIAL/30181 - analytical standard
Synonym: Acid Yellow 73; D&C;Yellow No. 8; NaFl; NaFluo; Sodium fluorescein; Uranine
CAS Number: 518-47-8
Empirical Formula (Hill Notation): C20H10Na2O5
Molecular Weight: 376.27
EC Number: 208-253-0
MDL Number: MFCD00167039
Linear Formula: C20H10Na2O5
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 30181-100MG
| assay | ≥97.5% (HPLC) |
| fluorescence | λex 460 nm; λem 515 nm(lit.) |
| format | neat |
| grade | analytical standard |
| impurities | ≤15% water |
| InChI | 1S/C20H12O5.2Na/c21-11-5- |
| InChI key | RGPLGPBQJOQFJS-UHFFFAOYSA |
| Quality Level | 100  |
| shelf life | limited shelf life, expiry date on the label |
| SMILES string | [Na+].[Na+].[O-]c1ccc2c(O |
| technique(s) | gas chromatography (GC): suitable |
| HPLC: suitable |
| Analysis Note: | remarks on ICP: Na positive |
| Application: | Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM). It may also be used as an analytical reference standard for the quantification of the analyte in the following: • Soil samples using high-performance liquid chromatography (HPLC). • Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE). |
| Application: | Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support. |
| General description: | Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97.5% (HPLC) |
| Colour Index Number | 45350 |
| UNSPSC | 85151701 |