Acridine Orange hydrochloride hydrate
SIAL/318337 - ≥98% (HPLC)
Synonym: 3,6-
CAS Number: 65-61-2
Empirical Formula (Hill Notation): C17H19N3 · HCl · xH2O
Molecular Weight: 301.81 (anhydrous basis)
EC Number: 200-614-0
MDL Number: MFCD00150070
Linear Formula: C17H19N3 · HCl · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 318337-250MG.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 318337-1G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 318337-5G
| ε (extinction coefficient) | ≥14,000 at 227-233 nm in water at 0.005 g/L |
| ≥35000 at 265-271 nm in water at 0.005 g/L | |
| λmax | 492 nm |
| application(s) | diagnostic assay manufacturing hematology histology |
| assay | ≥98% (HPLC) |
| color | orange |
| form | powder |
| InChI | 1S/C17H19N3.ClH.H2O/c1-19 |
| InChI key | JUAAUEXLEKAEQC-UHFFFAOYSA |
| mp | 284-287 °C (lit.) |
| Quality Level | 200  |
| SMILES string | Cl[H].[H]O[H].CN(C)c1ccc2 |
| solubility | H2O: 0.1%, clear, orange to red |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | A rigorously purified zinc-free material suitable for use in flow cytometry. |
| Application: | Acridine Orange hydrochloride hydrate dye has been used: • to stain intracellular acidic vesicles. • to measure lysosomal membrane permeability (LMP). • or autophagy detection. |
| Application: | Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models. |
| Biochem/physiol Actions: | Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence. |
| Other Notes: | Discover LiChropur reagents ideal for HPLC or LC-MS analysis |
| Packaging: | 1, 5 g in glass bottle |
| Packaging: | 250 mg in glass bottle |
| Symbol |  GHS08 |
| Signal word | Warning |
| Hazard statements | H341 |
| Precautionary statements | P201 - P202 - P280 - P308 + P313 - P405 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (HPLC) |
| mp | 284-287 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 46005 |
| UNSPSC | 12171500 |