Urea
SIAL/33247 - puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%
Synonym: Carbamide; Carbonyldiamide
CAS Number: 57-13-6
Empirical Formula (Hill Notation): CH4N2O
Molecular Weight: 60.06
EC Number: 200-315-5
MDL Number: MFCD00008022
Linear Formula: NH2CONH2
Product Type: Chemical
| agency | reag. Ph. Eur. |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| sulfate (SO42-): ≤10 mg/kg | |
| assay | ≥99% |
| 99-101% (calc. on dry substance) | |
| cation traces | Fe: ≤2 mg/kg |
| density | 1.335 g/mL at 25 °C (lit.) |
| form | solid |
| grade | ACS reagent |
| puriss. p.a. | |
| ign. residue | ≤0.01% (as SO4) |
| impurities | alkalic reac. substances, complies |
| ≤0.0005% heavy metals (as Pb) | |
| ≤0.05% ammonium (NH4) | |
| ≤0.1% biuret | |
| InChI | 1S/CH4N2O/c2-1(3)4/h(H4,2 |
| InChI key | XSQUKJJJFZCRTK-UHFFFAOYSA |
| loss | ≤1.0% loss on drying, 105°C, 1 h |
| mp | 132-135 °C |
| 132-135 °C (lit.) | |
| Quality Level | 200  |
| SMILES string | NC(N)=O |
| solubility | H2O: soluble 480 g/L at 20 °C |
| suitability | in accordance for appearance of solution |
| in accordance for identity |
| Application: | Urea has been used in the following studies: • Synthesis of SnO2 nanoparticles. • Synthesis of urethanes, ureides and semicarbazides. • Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain. • Protein electrophoretic studies. |
| Application: | Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. |
| General description: | Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds. |
| General description: | Urea is the most commonly used slow release nitrogen fertilizer. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99%; 99-101% (calc. on dry substance) |
| mp | 132-135 °C (lit.); 132-135 °C |
| Density | 1.335 g/mL at 25 °C (lit.) |
| UNSPSC | 12352100 |