Nitrate Reagent B
SIAL/39441 - suitable for microbiology
Synonym: Sulfanilic acid solution; 4-
CAS Number: 121-57-3
MDL Number: MFCD00007886
Product Type: Chemical
application(s) | agriculture clinical testing environmental food and beverages pharmaceutical |
microbiology | |
composition | acetic acid (30%), 1000 mL |
sulfanilic acid, 8.0 g | |
InChI | 1S/C6H7NO3S/c7-5-1-3-6(4- |
InChI key | HVBSAKJJOYLTQU-UHFFFAOYSA |
Quality Level | 100 ![]() |
shelf life | limited shelf life, expiry date on the label |
SMILES string | Nc1ccc(cc1)S(O)(=O)=O |
suitability | Enterobacter spp. |
Escherichia coli | |
Neisseria spp. | |
anaerobic bacteria | |
coliforms | |
technique(s) | microbe id | metabolite detection: suitable |
Application: | α-Naphthylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphthylamine to form a red azo dye. |
Symbol | ![]() |
Signal word | Danger |
Hazard statements | H314 |
Precautionary statements | P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 - P405 |
Hazard Codes | C |
Risk Statements | 34 |
Safety Statements | 26-36/37/39-45 |
RIDADR | UN 2790C 8 / PGIII |
WGK Germany | WGK 1 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
UNSPSC | 41171621 |