Amphotericin B trihydrate
SIAL/46006 - VETRANAL®, analytical standard
CAS Number: 1397-89-3 (anhydrous)
Empirical Formula (Hill Notation): C47H73NO17 · 3H2O
Molecular Weight: 978.12
EC Number: 215-742-2
MDL Number: MFCD25541905
Linear Formula: C47H73NO17 · 3H2O
Product Type: Chemical
application(s) | clinical testing |
format | neat |
grade | analytical standard |
InChI | 1S/C47H73NO17.3H2O/c1-27- |
InChI key | QEWGLCMWTBNETQ-KCPNHEJKSA |
product line | VETRANAL® |
Quality Level | 200 |
shelf life | limited shelf life, expiry date on the label |
SMILES string | O[C@]1(C[C@H](O)[C@@H](C( |
storage temp. | 2-8°C |
technique(s) | gas chromatography (GC): suitable |
HPLC: suitable |
Application: | Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium. |
Application: | Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support. |
Biochem/physiol Actions: | Mode of Action: Amphotericin B binds to sterols, forming channels in the membrane, and causing small molecules to leak out. Antimicrobial Spectrum: The product is most effective against fungi and yeast. |
Caution: | Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years. |
Legal Information: | VETRANAL is a registered trademark of Sigma-Aldrich Chemie GmbH |
Preparation Note: | Amphotericin B is insoluble in water at pH 6 to 7, but soluble in water at pH 2 or 11. It is soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL). Aqueous solutions cannot be sterile filtered due to poor solubility. |
Symbol | GHS08 |
Signal word | Danger |
Hazard statements | H372 |
Precautionary statements | P260 - P264 - P270 - P314 - P501 |
Hazard Codes | Xi |
Risk Statements | 36/37/38 |
Safety Statements | 26-36 |
RIDADR | NONH for all modes of transport |
WGK Germany | WGK 3 |
Flash Point(F) | Not applicable |
Flash Point(C) | Not applicable |
Storage Temp. | 2-8°C |
UNSPSC | 41116107 |