ONPG Disks
SIAL/49940 - suitable for microbiology
Synonym: β-Galactosidase Test Disks; 2-Nitrophenyl-β-D-galactopyranoside Disks
Product Type: Chemical
| agency | according to GB 4789.4-2016 |
| application(s) | environmental food and beverages |
| microbiology | |
| product line | BioChemika |
| Quality Level | 100  |
| shelf life | limited shelf life, expiry date on the label |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| suitability | Escherichia coli |
| bacteria | |
| technique(s) | microbe id | specific enzyme detection: suitable |
| General description: | ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters. |
| Packaging: | Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galacto |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41171621 |