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Sepharose™ 4B

SIAL/4B200 - 45-165 μm bead diameter

CAS Number: 9012-36-6
MDL Number: MFCD08457579
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-4B200-100ML 100 mL
 $266.00
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45-4B200-500ML 500 mL
 $978.00
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This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 4B200-500ML.

 

bead diameter 45-165 μm
biological source algae (marine)
form suspension
InChI 1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key MJQHZNBUODTQTK-WKGBVCLCSA-N
pore size 30,000-50,000 Da fractionation range (Dextrans)
  60,000-20,000,000 Da fractionation range (Globular proteins)
Quality Level 200 
SMILES string O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
storage temp. 2-8°C
technique(s) affinity chromatography: suitable
Application: Sepharose™ 4B has been used:
• to conjugate scorpion venom to be used in affinity chromatography
• for clearing of protein extracts prior to immunoprecipitation
• for gel purification and desalting of phospholipid vesicles
• for adsorption of immunoglobulin G (IgG)
General description: Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.
Legal Information: Sepharose is a trademark of Cytiva
Packaging: 1 L in glass bottle
Packaging: 100, 500 mL in glass bottle
Storage Temp. 2-8°C
UNSPSC 12352200

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