Methylene Blue solution
SIAL/50484 - suitable for microbiology
Synonym: Ehrlich’s reagent III; Loeffler’s Methylene Blue
CAS Number: 61-73-4
Empirical Formula (Hill Notation): C16H18ClN3S
Molecular Weight: 319.85
MDL Number: MFCD00012111
Linear Formula: C16H18ClN3S
Product Type: Chemical
| application(s) | microbiology |
| concentration | 0.5% in H2O (0.5 g/100 mL) |
| InChI | 1S/C16H18N3S.ClH/c1-18(2) |
| InChI key | CXKWCBBOMKCUKX-UHFFFAOYSA |
| shelf life | limited shelf life, expiry date on the label |
| SMILES string | [Cl-].CN(C)c1ccc2N=C3C=C |
| suitability | Bacillus spp. |
| Corynebacterium spp. | |
| bacteria | |
| technique(s) | microbe id | staining: suitable |
| Components: | reagent contains: methylene blue 0.5 g distilled water 100 ml |
| General description: | Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925). |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12171500 |