Ethanol
SIAL/51976 - BioUltra, Molecular Biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)
Synonym: Ethyl alcohol
CAS Number: 64-17-5
Empirical Formula (Hill Notation): C2H6O
Molecular Weight: 46.07
EC Number: 200-578-6
MDL Number: MFCD00003568
Linear Formula: CH3CH2OH
Product Type: Chemical
| λ | neat |
| application(s) | life science and biopharma |
| assay | ≥99.8% |
| autoignition temp. | 683 °F |
| bp | 78 °C (lit.) |
| cation traces | Al: ≤0.5 mg/kg |
| Ba: ≤0.1 mg/kg | |
| Bi: ≤0.1 mg/kg | |
| Ca: ≤0.5 mg/kg | |
| Cd: ≤0.05 mg/kg | |
| Co: ≤0.02 mg/kg | |
| Cr: ≤0.02 mg/kg | |
| Cu: ≤0.1 mg/kg | |
| Fe: ≤0.1 mg/kg | |
| K: ≤0.5 mg/kg | |
| Li: ≤0.1 mg/kg | |
| Mg: ≤0.1 mg/kg | |
| Mn: ≤0.02 mg/kg | |
| Mo: ≤0.1 mg/kg | |
| Na: ≤1 mg/kg | |
| Ni: ≤0.02 mg/kg | |
| Pb: ≤0.1 mg/kg | |
| Sr: ≤0.1 mg/kg | |
| Zn: ≤0.1 mg/kg | |
| evapn. residue | ≤0.001% |
| expl. lim. | 19 %, 60 °F |
| form | liquid |
| format | neat |
| grade | Molecular Biology |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| KMnO4-reducing substances, in accordance | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| substances darkened by H2SO4, in accordance | |
| ≤0.0004% free alkali (as NH3) | |
| ≤0.001% aldehyde (as CH3CHO) | |
| ≤0.001% formaldehyde (as HCHO) | |
| ≤0.001% ketone (as CH3COCH3) | |
| ≤0.002% free acid (as CH3COOH) | |
| ≤0.1% methanol (CH3OH) | |
| ≤0.2% water | |
| InChI | 1S/C2H6O/c1-2-3/h3H,2H2,1 |
| InChI key | LFQSCWFLJHTTHZ-UHFFFAOYSA |
| mp | −114 °C (lit.) |
| product line | BioUltra |
| quality | (absolute alcohol, without additive, A15 o1) |
| Quality Level | 200 ![]() |
| refractive index | n |
| n |
|
| SMILES string | CCO |
| solubility | H2O: miscible |
| organic solvents: miscible | |
| suitability | suitable for molecular biology |
| UV absorption | λ: 260 nm Amax: 0.04 |
| λ: 280 nm Amax: 0.03 | |
| vapor density | 1.59 (vs air) |
| vapor pressure | 44.6 mmHg ( 20 °C) |
| Application: | Ethanol has been used: • in vehicle-treated controls to perform mouse experiments • in viability assay along with crystal violet to stain human and mouse high-grade serous ovarian cancer (HGSOC) cells after stimulation • to prepare 17β-estradiol (E2) to use in cell culture experiments • in cell cycle analysis for fixing cells drop-wise |
| Biochem/physiol Actions: | Ethanol is a widely used organic chemical in industry and consumer goods. This aliphatic alcohol is mainly used in industry as a solvent and as an intermediate in the production of other chemicals. |
| Features and Benefits: | • Versatile and adaptable for a wide variety of laboratory and research applications • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations |
| General description: | Ethanol, also known as Ethyl Alcohol, is a volatile primary alcohol with a straight-chain structure. It is frequently employed as a biochemical reagent, effectively denaturing proteins from bacteria, fungi, and viruses. Widely utilized in cell biology, molecular biology, and biochemical research, this DNase, RNase, NICKase, and protease-free form of ethanol plays a crucial role in purifying and precipitating biomolecules like nucleic acids (DNA & RNA) and proteins. Additionally, it finds application in histology for preparing staining and destaining reagents, as well as in the dehydration of tissues before embedding. |
| Other Notes: | 1Distribution and use of ethyl alcohol in Switzerland require a license from the Swiss Federal Alcohol Administration |
| Other Notes: | For additional information on our range of Biochemicals , please complete this form . |
| Other Notes: | Sales restrictions may apply. Suitable for the precipitation of nucleic acids |
| Symbol | ![]() GHS02,GHS07 |
| Signal word | Danger |
| Hazard statements | H225 - H319 |
| Precautionary statements | P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338 |
| Hazard Codes | F |
| Risk Statements | 11 |
| Safety Statements | 7-16 |
| RIDADR | UN1170 - class 3 - PG 2 - Ethanol |
| WGK Germany | WGK 1 |
| Flash Point(F) | 55.4 °F |
| Flash Point(C) | 13 °C |
| Purity | ≥99.8% |
| bp | 78 °C (lit.) |
| mp | −114 °C (lit.) |
| Refractive Index | n |
| UNSPSC | 12191601 |



