Brain Heart Infusion Broth
SIAL/53286 - suitable for microbiology, NutriSelect® Plus
Synonym: BHI Broth; Brain Heart Broth
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change.
Powder Culture Medium
| agency | according to ISO 6888-1 2020 |
| according to ISO 6888-3:2003 | |
| application(s) | clinical testing environmental food and beverages life science and biopharma pathogen testing pharmaceutical |
| microbiology | |
| composition | |
| beef heart (infusion from 250g), 5 g/L | |
| calf brains (infusion from 200g), 12.5 g/L | |
| disodium hydrogen phosphate, 2.5 g/L | |
| peptone, 10 g/L | |
| sodium chloride, 5 g/L | |
| final pH | 7.4±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | Haemophilus spp. |
| nonselective for Bacteroides spp. | |
| nonselective for Candida spp. | |
| nonselective for Escherichia coli | |
| nonselective for Neisseria spp. | |
| nonselective for Pseudomonas spp. | |
| nonselective for Staphylococcus spp. | |
| nonselective for Streptococcus spp. | |
| nonselective for Yersinia spp. | |
| nonselective for bacteria (General Media) | |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms. Brain Heart Infusion Broth has been used as culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P |
| Preparation Note: | Dissolve 37 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171607 |