Kovac’s reagent for indoles
SIAL/60983 - suitable for microbiology
Synonym: 4-
CAS Number: 100-10-7
Empirical Formula (Hill Notation): C9H11NO
Molecular Weight: 149.19
MDL Number: MFCD00003381
Linear Formula: C9H11NO
Product Type: Chemical
application(s) | agriculture clinical testing environmental food and beverages |
microbiology | |
composition | 4-dimethylaminobenzaldehy |
hydrochloric acid | |
n-butanol | |
InChI | 1S/C9H11NO/c1-10(2)9-5-3- |
InChI key | BGNGWHSBYQYVRX-UHFFFAOYSA |
Quality Level | 100 ![]() |
refractive index | n |
shelf life | limited shelf life, expiry date on the label |
SMILES string | [H]C(=O)c1ccc(cc1)N(C)C |
storage temp. | 2-8°C |
suitability | Escherichia coli |
coliforms | |
technique(s) | microbe id | specific enzyme detection: suitable |
Application: | Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater. |
Biochem/physiol Actions: | Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test. |
General description: | Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzalde |
Symbol | ![]() ![]() ![]() |
Signal word | Danger |
Hazard statements | H226 - H290 - H302 - H315 - H318 - H335 - H336 |
Precautionary statements | P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
Hazard Codes | Xn |
Risk Statements | 10-22-37/38-41-67 |
Safety Statements | 26-39 |
RIDADR | UN 2920 3(8) / PGII |
WGK Germany | WGK 1 |
Flash Point(F) | 96.8 °F |
Flash Point(C) | 36 °C |
Refractive Index | n |
Storage Temp. | 2-8°C |
UNSPSC | 41171621 |