Kovac’s reagent for indoles

SIAL/60983 - suitable for microbiology

Synonym: 4-(Dimethylamino)benzaldehyde solution; Indole reagent according to Kovac

CAS Number: 100-10-7
Empirical Formula (Hill Notation): C₉H₁₁NO
Molecular Weight: 149.19
MDL Number: MFCD00003381
Linear Formula: C₉H₁₁NO
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-60983-100ML	100 mL	$112.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

application(s)	agriculture clinical testing environmental food and beverages
	microbiology
composition	4-dimethylaminobenzaldehyde
	hydrochloric acid
	n-butanol
InChI	1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
InChI key	BGNGWHSBYQYVRX-UHFFFAOYSA-N
Quality Level	100
refractive index	n20/D 1.400-1.420
shelf life	limited shelf life, expiry date on the label
SMILES string	[H]C(=O)c1ccc(cc1)N(C)C
storage temp.	2-8°C
suitability	Escherichia coli
	coliforms
technique(s)	microbe id \| specific enzyme detection: suitable

Application:	Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.
Biochem/physiol Actions:	Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.
General description:	Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Symbol	GHS02,GHS05,GHS07
Signal word	Danger
Hazard statements	H226 - H290 - H302 - H315 - H318 - H335 - H336
Precautionary statements	P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
Hazard Codes	Xn
Risk Statements	10-22-37/38-41-67
Safety Statements	26-39
RIDADR	UN 2920 3(8) / PGII
WGK Germany	WGK 1
Flash Point(F)	96.8 °F
Flash Point(C)	36 °C

Refractive Index	n20/D 1.400-1.420
Storage Temp.	2-8°C
UNSPSC	41171621

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