Crystal Violet
SIAL/61135 - for microscopy (Bact., Bot., Hist., Vit.), indicator (pH 0.1-2.0)
Synonym: Basic Violet 3; Gentian Violet; Hexamethylpararosaniline chloride; Methyl Violet 10B
CAS Number: 548-62-9
Empirical Formula (Hill Notation): C25H30ClN3
Molecular Weight: 407.98
MDL Number: MFCD00011750
Linear Formula: C25H30N3Cl
Product Type: Chemical
| ε (extinction coefficient) | ≥1750 at 585-595 nm in water |
| antibiotic activity spectrum | fungi |
| application(s) | diagnostic assay manufacturing hematology histology |
| density | 1.190 g/cm3 |
| form | powder |
| grade | for microscopy (Bact., Bot., Hist., Vit.) |
| indicator (pH 0.1-2.0) | |
| InChI | 1S/C25H30N3.ClH/c1-26(2)2 |
| InChI key | ZXJXZNDDNMQXFV-UHFFFAOYSA |
| mode of action | cell membrane | interferes |
| enzyme | inhibits | |
| mp | 205 °C (dec.) (lit.) |
| Quality Level | 200  |
| SMILES string | [Cl-].CN(C)c1ccc(cc1)C(c |
| storage temp. | room temp |
| suitability | suitable for microscopy (Bact., Bot., Hist., Vit.) |
| visual transition interval | 0.1-2.0, yellow-green to blue-violet |
| Application: | Crystal violet is commonly used in the Gram stain and its variants; and to stain amyloid, bacterial components, and vascular plant tissues. It is also used for the staining of plant chromosomes.Crystal violet is also suitable for DNA visualization in agarose gels. The dye is used only in the presence of high concentrations of DNA. Crystal Violet also helps in the colorimetric measurement of cell viability. |
| General description: | Crystal violet is used as an active component, primary stain, of Gram stain for differentiation of Gram-negative versus Gram-positive bacteria. It is a hexa-N-methylated triaminotriphenylmethane with a lipophilic cation. It possesses a slightly nonplanar conjugated system. |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H302 - H318 - H341 - H350 - H410 |
| Precautionary statements | P201 - P273 - P280 - P301 + P312 - P305 + P351 + P338 - P308 + P313 |
| Hazard Codes | T,N |
| Risk Statements | 45-22-41-50/53-68 |
| Safety Statements | 53-26-36/37/39-45-61 |
| RIDADR | UN 3077 9 / PGIII |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 205 °C (dec.) (lit.) |
| Density | 1.190 g/cm3 |
| Storage Temp. | room temp |
| Colour Index Number | 42555 |
| UNSPSC | 12171500 |