M9 Broth
SIAL/63011 - suitable for microbiology, NutriSelect® Basic
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 63011-500G-F
| application(s) | food and beverages life science and biopharma |
| microbiology | |
| composition | ammonium chloride, 1 g/L |
| disodium hydrogen phosphate, 6 g/L | |
| potassium dihydrogen phosphate, 3 g/L | |
| sodium chloride, 0.5 g/L | |
| final pH | 7.4±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Basic |
| Quality Level | 100  |
| sterility | non-sterile |
| suitability | nonselective for Escherichia coli |
| nonselective for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | M9 Broth is used as a microbiological growth media and for the preparation of phage and plasmid DNA using E. coli K12 strains, acc. to Miller (1987). |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Dissolve 10.5 g in 1 litre distilled water and adjust the pH to 7.4. Sterilize by autoclaving at 121°C for 15 minutes. Add 2 ml 1 M magnesium sulfate (Cat. No. 63133; filter sterilized) and 20 ml of 20% solution from a carbon source (such as glucose; Cat. No. 49140) |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171607 |