5-Maleimido-eosin
SIAL/63184 - for fluorescence, ≥93% (HPLC)
Synonym: EMA; Eosin-5-maleimide; Eosin-5-maleimide
CAS Number: 150322-02-4
Empirical Formula (Hill Notation): C24H9Br4NO7
Molecular Weight: 742.95
MDL Number: MFCD07437936
Linear Formula: C24H9Br4NO7
Product Type: Chemical
| assay | ≥93% (HPLC) |
| fluorescence | λex 524 nm; λem 545 nm (after derivatization with 2-mercaptoethanol) |
| grade | for fluorescence |
| InChI | 1S/C24H9Br4NO7/c25-13-6-1 |
| InChI key | YRCLWXRIEYQTAV-UHFFFAOYSA |
| Quality Level | 100  |
| SMILES string | Oc1c(Br)cc2c(Oc3c(Br)c(O) |
| storage temp. | 2-8°C |
| Application: | Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK). |
| General description: | 5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading. |
| Other Notes: | Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥93% (HPLC) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |