Methylene Blue hydrate
SIAL/66720 - purum p.a., suitable for microscopy, ≥97.0% (calc. based on dry substance, AT)
CAS Number: 122965-43-9
Empirical Formula (Hill Notation): C16H18ClN3S · xH2O
Molecular Weight: 319.85 (anhydrous basis)
EC Number: 200-515-2
MDL Number: MFCD00150006
Linear Formula: C16H18ClN3S · xH2O
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 66720-100G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 66720-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 66720-500G
| λmax | 656-666 nm in water |
| assay | ≥97.0% (calc. based on dry substance, AT) |
| color | green to dark green |
| concentration | ≤100% (Methylthioninium chloride hydrate) |
| form | powder |
| grade | purum p.a. |
| InChI | 1S/C16H18N3S.ClH.H2O/c1-1 |
| InChI key | WQVSELLRAGBDLX-UHFFFAOYSA |
| mol wt | 319.85 g/mol (anhydrous basis) |
| Quality Level | 200  |
| SMILES string | O.[Cl-].CN(C)c1ccc2N=C3C= |
| solubility | ethanol: 0.01 g/10 mL, blue to very deep blue |
| storage temp. | room temp |
| suitability | suitable for microscopy |
| Application: | Methylene Blue hydrate has been used: • in the preparation of methylene blue (MB) solution to stain zymograms • to label the injection site in the tissue slices • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss |
| Biochem/physiol Actions: | Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment. MB can be considered as a possible and safe vasoconstrictor. It has several applications in textile, paper and hair coloring sectors. |
| General description: | Methylene Blue (MB) is a basic histological dye. |
| Other Notes: | may contain up to 22% water |
| Other Notes: | On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis |
| Packaging: | 25, 100, 500 g in poly bottle |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H302 |
| Hazard Codes | Xn |
| Risk Statements | 22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥97.0% (calc. based on dry substance, AT) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |