Kovac’s reagent for indoles

SIAL/67309 - suitable for microbiology

Synonym: 4-(Dimethylamino)benzaldehyde solution

CAS Number: 100-10-7
Empirical Formula (Hill Notation): C₉H₁₁NO
Molecular Weight: 149.19
MDL Number: MFCD00003381
Linear Formula: C₉H₁₁NO
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-67309-100ML-F	100 mL	$102.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

agency	according to ISO 16654:2001
application(s)	agriculture clinical testing environmental food and beverages
	microbiology
composition	4-(dimethlyamino)benzaldehyde, 50 g/L
	hydrochloric acid, 240 g/L
	isoamylic alcohol, 710 g/L
InChI	1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
InChI key	BGNGWHSBYQYVRX-UHFFFAOYSA-N
product line	BioChemika
Quality Level	200
shelf life	limited shelf life, expiry date on the label
SMILES string	[H]C(=O)c1ccc(cc1)N(C)C
storage temp.	2-8°C
suitability	Escherichia coli
	coliforms
technique(s)	microbe id \| specific enzyme detection: suitable

Application:	In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.
Application:	Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.
General description:	Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Symbol	GHS02,GHS05,GHS07
Signal word	Danger
Hazard statements	H226 - H290 - H314 - H317 - H332 - H335
Precautionary statements	P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Codes	C
Risk Statements	10-20-34-37
Safety Statements	26-36/37/39-45
RIDADR	UN 2920 3(8) / PGII
WGK Germany	WGK 1
Flash Point(F)	109.4 °F
Flash Point(C)	43 °C

Storage Temp.	2-8°C
UNSPSC	41171621

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