Tris Acetate-EDTA buffer Concentrate
SIAL/67996 - for Northern and Southern blotting, solution
Product Type: Chemical
| density | 1.09 g/mL at 20 °C |
| form | solution |
| grade | for molecular biology |
| impurities | DNases, none detected |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| pH | 8.3±0.2 (25 °C) |
| quality | 50x Concentrate |
| storage temp. | room temp |
| Application: | Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations. |
| Components: | The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water. |
| General description: | TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Density | 1.09 g/mL at 20 °C |
| Storage Temp. | room temp |
| UNSPSC | 12161700 |