Fraser Broth, Base
SIAL/69198 - suitable for microbiology, NutriSelect® Plus
Synonym: Listeria Selective Enrichement Broth, Base (acc. Fraser)
Product Type: Chemical
Microbial culture media preparation
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 69198-500G
| application(s) | agriculture environmental food and beverages pathogen testing sample preparation |
| microbiology | |
| composition | casein enzymic hydrolysate, 5.0 g/L |
| disodium phosphate dihydrate, 12.0 g/L | |
| esculin, 1.0 g/L | |
| lithium chloride, 3.0 g/L | |
| meat extract, 5.0 g/L | |
| peptic digest of animal tissue, 5.0 g/L | |
| potassium dihydrogen phosphate, 1.35 g/L | |
| sodium chloride, 20.0 g/L | |
| yeast extract, 5.0 g/L | |
| final pH | 7.2±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective and differential for Listeria spp. |
| technique(s) | microbiological culture: suitable |
| Application: | Fraser Broth Base with added supplement is recommended by ISO Committee for primary and secondary enrichment medium, for the isolation and enumeration of Listeria monocytogenes from food and animal feeds |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 57.35 grams of dehydrated medium in 1000 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Fraser Selective Supplement (Cat. No. 18038) and 2 vials of Fraser Supplement (Cat. No. 90836) to 1000 ml medium for primary enrichment or 1 vial of each to 500 ml medium for secondary enrichment. Mix well and dispense as desired. Warning : Lithium chloride is harmful. Avoid bodily contact and inhalation of vapours. On contact with skin wash with plenty of water immediately. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |