MES monohydrate
SIAL/69889 - BioUltra, for molecular biology, ≥99.5% (T)
Synonym: 2-
CAS Number: 145224-94-8
Empirical Formula (Hill Notation): C6H13NO4S · H2O
Molecular Weight: 213.25
EC Number: 224-632-3
MDL Number: MFCD00149409
Linear Formula: C6H13NO4S · H2O
Product Type: Chemical
| λ | 0.5 M in H2O |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.5% (T) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤20 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | powder or crystals |
| grade | for molecular biology |
| ign. residue (900 °C) | ≤0.05% (as SO4) |
| impurities | DNases, none detected |
| Insoluble matter, passes filter test | |
| Phosphatases, none detected | |
| Proteases, none detected | |
| RNases, none detected | |
| InChI | 1S/C6H13NO4S.H2O/c8-12(9, |
| InChI key | MIIIXQJBDGSIKL-UHFFFAOYSA |
| mp | >300 °C (lit.) |
| pH | 2.5-4.0 (25 °C, 0.5 M in H2O) |
| pKa (25 °C) | 6.1 |
| product line | BioUltra |
| Quality Level | 200  |
| SMILES string | O.OS(=O)(=O)CCN1CCOCC1 |
| solubility | H2O: 0.5 M at 20 °C, clear, colorless |
| useful pH range | 5.5-6.7 |
| UV absorption | λ: 260 nm Amax: 0.025 |
| λ: 280 nm Amax: 0.020 |
| Application: | MES Monohydrate has been used: • In the preparation of a solution used for the suspension and dilution of protoplasts to measure the density and number of protoplasts using a haemocytometer • As a component of Murashige and Skoog basal salt media • In the preparation of total ionic strength adjustment buffer (TISAB) |
| General description: | MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in molecular biology. |
| Other Notes: | Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5 M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8C for months. Sterilization: Sterilization should be by filteration through 0.2 uM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, and then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown. |
| Preparation Note: | A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published. |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.5% (T) |
| mp | >300 °C (lit.) |
| UNSPSC | 12161700 |