EMB Agar
SIAL/70186 - NutriSelect® Plus, suitable for microbiology
Synonym: Eosin Methylene Blue Agar
Product Type: Chemical
Versatile solid medium for the differentiation of Eschericha coli
| application(s) | agriculture cleaning products clinical testing cosmetics environmental food and beverages personal care |
| microbiology | |
| composition | agar, 15.0 g/L |
| dipotassium hydrogen phosphate, 2.0 g/L | |
| eosin Y, 0.4 g/L | |
| lactose, 10.0 g/L | |
| methylene blue, 0.065 g/L | |
| peptone, 10.0 g/L | |
| final pH | 7.1±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | slightly selective and differential for Candida spp. |
| slightly selective and differential for Citrobacter spp. | |
| slightly selective and differential for Enterobacter spp. | |
| slightly selective and differential for Enterococcus spp. | |
| slightly selective and differential for Escherichia coli | |
| slightly selective and differential for Klebsiella spp. | |
| slightly selective and differential for Proteus spp. | |
| slightly selective and differential for Salmonella spp. | |
| slightly selective and differential for Staphylococcus spp. | |
| slightly selective and differential for coliforms | |
| slightly selective and differential for enterobacteriaceae | |
| technique(s) | microbiological culture: suitable |
| Application: | A versatile solid medium for the differentiation of Eschericha coli and Aerobacter aerogenes, for identifcation of Candida albicans and for the identification of coagulase-positive Staphylococci. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Recommended by the American Public Health Association (APHA) and the United States Pharmacopeia (USP); Compendium of Methods for the Microbiological Examination of Foods; Enumeration of E. coli from lake water. |
| Preparation Note: | Suspend 37.5 g in 1 litre of distilled water. Bring to the boil to dissolve completely, sterilize by autoclaving at 121°C for 15 minutes. Cool to 60°C and shake the medium in order to oxidize the methylene blue and to suspend the precipitate. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |