Violet Red Bile Agar
SIAL/70188 - suitable for microbiology, NutriSelect® Plus
Synonym: VRB Agar; VRBL-Agar
Product Type: Chemical
Detection and enumeration of coliform organisms
| agency | according to ISO 4832:2006 |
| application(s) | agriculture bioburden testing environmental food and beverages water monitoring |
| microbiology | |
| composition | agar, 12.0 g/L |
| bile salts, 1.5 g/L | |
| crystal violet, 0.002 g/L | |
| lactose, 10.0 g/L | |
| neutral red, 0.03 g/L | |
| peptone, 7.0 g/L | |
| sodium chloride, 5.0 g/L | |
| yeast extract, 3.0 g/L | |
| final pH | 7.4±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| suitability | selective and differential for Enterobacter spp. |
| selective and differential for Escherichia coli | |
| selective and differential for coliforms | |
| technique(s) | microbiological culture: suitable |
| Application: | A selective medium for the detection and enumeration of coliform organisms, especially the Coli-aerogenes-group in water, milk and other dairy products. |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Recommended by the American Public Health Association (APHA); Studies on coliform bacteria |
| Preparation Note: | Suspend 38.5 g in 1 litre of distilled water and let it sock for 5 minutes. Bring to boil and distribute into plates or tubes. Sterilization at 121°C for 15 minutes is optional, but if the medium is to be used on the same day of preparation it needs to be sterilized. But through sterilization may the medium is going to be turbid. No sterilization improves the recovery of the stressed microorganisms. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 41171606 |