Mueller Hinton Broth
SIAL/70192 - suitable for microbiology, NutriSelect® Plus
Synonym: MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media
Product Type: Chemical
| application(s) | clinical testing food and beverages |
| microbiology | |
| composition | beef infusion solids, 2.0 g/L |
| casein hydrolysate, 17.5 g/L | |
| starch, 1.5 g/L | |
| final pH | 7.4±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| product line | BioChemika |
| Quality Level | 200  |
| shelf life | limited shelf life, expiry date on the label |
| sterility | non-sterile |
| storage condition | protect from light |
| storage temp. | 15-25°C |
| suitability | nonselective for bacteria (General Media) |
| technique(s) | microbe id | susceptibility testing: suitable |
| microbiological culture: suitable |
| Application: | Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 15-25°C |
| UNSPSC | 41171504 |