α-Cyano-4-hydroxycinnamic acid
SIAL/70990 - matrix substance for MALDI-MS, ≥99.0% (HPLC)
Synonym: α-CCA; α-CHCA; α-Cyano; 4-HCCA; ACCA
CAS Number: 28166-41-8
Empirical Formula (Hill Notation): C10H7NO3
Molecular Weight: 189.17
EC Number: 248-879-1
MDL Number: MFCD00004204
Linear Formula: HOC6H4CH=C(CN)CO2H
Product Type: Chemical
| absorption | ≥2000 at 337 nm (mol. absorption) |
| analyte chemical class(es) | glycans, peptides, proteins |
| assay | ≥99.0% (HPLC) |
| cation traces | Ba: ≤5 mg/kg |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| K: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Na: ≤10 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| form | solid |
| grade | matrix substance for MALDI-MS |
| InChI | 1S/C10H7NO3/c11-6-8(10(13 |
| InChI key | AFVLVVWMAFSXCK-VMPITWQZSA |
| mp | 245-250 °C |
| 245-250 °C (lit.) | |
| Quality Level | 100  |
| SMILES string | OC(=O)C(=Cc1ccc(O)cc1)C |
| solubility | methanol: 10 mg/mL, clear |
| technique(s) | MALDI-MS: suitable |
| Application: | α-CHCA was used as matrix in improving the detection of low concentration protein digests on MALDI-TOF. |
| General description: | α-Cyano-4-hydroxycinnamic acid (α-CHCA) is widely used as a matrix in order to facilitate ionization of peptides and proteins in a matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometer. |
| Other Notes: | Matrix substance used in MALDI-MS (matrix-assisted laser desorption ionization MS) peptide analysis in proteomics. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H317 - H319 - H335 |
| Precautionary statements | P280 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥99.0% (HPLC) |
| mp | 245-250 °C (lit.); 245-250 °C |
| UNSPSC | 12000000 |