modified Tryptic Soy Broth- Dehydrated Culture Media
SIAL/76704 - NutriSelect® Plus, novobiocin, according to ISO 16654:2001, powder, selective for enterovirulent E. coli (EEC)
Synonym: Modified Tryptic Soy Broth with Novobiocin; Modified Tryptic Soy Broth; EHEC enrichment broth; mTSB Broth with Novobiocin
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 76704-500G-F
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: 76704-500G-F
| agency | according to ISO 16654:2001 |
| application(s) | agriculture food and beverages microbiology pathogen testing |
| color | faint brown |
| composition | |
| bile salts No. 3, 1.5 g/L | |
| di-potassium hydrogen phosphate, 4.0 g/L | |
| novobiocin | |
| peptone from casein, 17.0 g/L | |
| peptone from soymeal, 3.0 g/L | |
| sodium chloride, 5.0 g/L | |
| final pH | 7.3±0.2 (25 °C) |
| form | powder |
| manufacturer/tradename | NutriSelect® Plus |
| packaging | pkg of -500 g |
| Quality Level | 200  |
| shelf life | limited, expiry date on the label |
| shipped in | wet ice |
| sterility | non-sterile |
| storage condition | (Dry place, tightly sealed containers) |
| storage temp. | 2-8°C |
| suitability | selective for Escherichia coli |
| selective for coliforms | |
| selective for enterovirulent E. coli (EEC) | |
| technique(s) | microbiological culture: suitable |
| Application: | mTSB Broth with Novobiocin is recommended for the selective enrichment of enterohemorrhagic E. coli (EHEC) in foods, especially meat and poultry products.The medium meets the requirements of the DIN Norm 10167 for the detection of E. coli serotype O157:H7 in foods. It is also recommended by the FDA-BAM as a method for the isolation of enterohemorrhagic E. coli (EHEC). |
| Footnote: | We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance. |
| General description: | mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation. |
| Legal Information: | GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Legal Information: | NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Preparation Note: | Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41171607 |