Methylene Blue solution
SIAL/77515 - for microscopy
Synonym: Ehrlich’s reagent III; Loeffler’s Methylene Blue
CAS Number: 61-73-4
Empirical Formula (Hill Notation): C16H18ClN3S
Molecular Weight: 319.85
MDL Number: MFCD00012111
Linear Formula: C16H18ClN3S
Product Type: Chemical
antibiotic activity spectrum | Gram-negative bacteria |
Gram-positive bacteria | |
form | solution |
grade | for microscopy |
InChI | 1S/C16H18N3S.ClH/c1-18(2) |
InChI key | CXKWCBBOMKCUKX-UHFFFAOYSA |
shelf life | limited shelf life, expiry date on the label |
SMILES string | [Cl-].CN(C)c1ccc2N=C3C=C |
suitability | Corynebacterium spp. |
bacteria | |
mycobacteria | |
spore forming microorganisms | |
technique(s) | microbe id | staining: suitable |
Application: | Loefflers Methylene Blue stain is used for the identification of diphteria bacilli, since it differentiates the deeply staining metachromatic granules from the pale blue-staining cytoplasm. |
Components: | Composition: methylene blue 0.3 g ethanol 30 ml water 100 ml |
Other Notes: | This product has been replaced by 03978-SIGMA | Methylene Blue solution for microscopy, concentrate according to Ehrlich, concentrated, aqueous solution ![]() |
Symbol | ![]() |
Signal word | Warning |
Hazard statements | H226 |
Risk Statements | 10 |
RIDADR | NA 1993 / PGIII |
WGK Germany | WGK 2 |
Flash Point(F) | 113.0 °F |
Flash Point(C) | 45 °C |
UNSPSC | 12171500 |