Phenol
SIAL/77608 - BioUltra, for molecular biology, ≥99.5% (GC)
Synonym: Hydroxybenzene
CAS Number: 108-95-2
Empirical Formula (Hill Notation): C6H6O
Molecular Weight: 94.11
EC Number: 203-632-7
MDL Number: MFCD00002143
Linear Formula: C6H5OH
Product Type: Chemical
| absorption | cut-off at 294 nm in H2O at 0.5 M |
| anion traces | chloride (Cl-): ≤5 mg/kg |
| sulfate (SO42-): ≤50 mg/kg | |
| assay | ≥99.5% (GC) |
| autoignition temp. | 1319 °F |
| bp | 182 °C (lit.) |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| contains | ~0.15% hypophosphorous acid as stabilizer |
| density | 1.071 g/mL at 25 °C (lit.) |
| expl. lim. | 8.6 % |
| form | solid |
| grade | for molecular biology |
| ign. residue | ≤0.05% |
| impurities | DNases, none detected |
| insoluble matter, passes filter test | |
| phosphatases, none detected | |
| proteases, none detected | |
| RNases, none detected | |
| ≤0.5% water | |
| InChI | 1S/C6H6O/c7-6-4-2-1-3-5-6 |
| InChI key | ISWSIDIOOBJBQZ-UHFFFAOYSA |
| mp | 40-42 °C |
| 40-42 °C (lit.) | |
| pH | 3.0-6.0 (25 °C, 0.5 M in H2O) |
| product line | BioUltra |
| Quality Level | 100  |
| SMILES string | Oc1ccccc1 |
| solubility | H2O: 0.5 M at 20 °C, clear, colorless |
| storage temp. | 2-8°C |
| suitability | suitable for molecular biology |
| vapor density | 3.24 (vs air) |
| vapor pressure | 0.09 psi ( 55 °C) |
| 0.36 mmHg ( 20 °C) |
| Application: | Phenol has been used in the incubation of pellet for RNA extraction. |
| Biochem/physiol Actions: | Phenol has the ability to denature protein, hence can lead to denervation. At lower concentration, it can serve as a local anaesthetic and can also act as a neurolytic agent in higher concentration. It is also linked with tissue damage at higher concentrations. |
| General description: | Phenol/carbolic acid is mainly produced by hospital pharmacy as it is unavailable in premixed liquid form. It is not highly soluble in water. Phenol should be refrigerated and protected from light exposure to maintain its shelf life of approximately one year. Kidneys can excrete phenol as several conjugated derivatives. |
| Other Notes: | For the preparation of DNA ; Large- and small-scale phenol extractions |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H311 + H331 - H314 - H341 - H373 - H411 |
| Precautionary statements | P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | T |
| Risk Statements | 23/24/25-34-48/20/21/22-68 |
| Safety Statements | 24/25-26-28-36/37/39-45 |
| RIDADR | UN 1671 6.1 / PGII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 174.2 °F - closed cup |
| Flash Point(C) | 79.0 °C - closed cup |
| Purity | ≥99.5% (GC) |
| bp | 182 °C (lit.) |
| mp | 40-42 °C (lit.); 40-42 °C |
| Density | 1.071 g/mL at 25 °C (lit.) |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352104 |