Phenol – chloroform – isoamyl alcohol mixture

SIAL/77619 - BioUltra, for molecular biology, 125:24:1

CAS Number: 136112-00-0
MDL Number: MFCD00133763
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-77619-100ML	100 mL	$108.00 1/EA		Add To Favorites
45-77619-500ML	500 mL	$376.00 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ	neat
density	1.12 g/mL at 20 °C
form	liquid
grade	for molecular biology
impurities	DNases, none detected
	phosphatases, none detected
	proteases, none detected
	RNases, none detected
InChI	1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H
InChI key	ZYWFEOZQIUMEGL-UHFFFAOYSA-N
pH	4-5(H₂O-phase, after extraction with H₂O, 1:1)
product line	BioUltra
Quality Level	100
SMILES string	ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1
storage temp.	2-8°C
UV absorption	λ: 330 nm A_max: 0.2
	λ: 405 nm A_max: 0.1
	λ: 510 nm A_max: 0.1

Application:

Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems: This research explores the application of the Phenol – chloroform – isoamyl alcohol mixture in extracting high-quality RNA for advanced therapeutic formulations, emphasizing its critical role in nanotechnology and pharmaceutical sciences (Lokras et al., 2022 ).

Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry: Demonstrates the use of the Phenol – chloroform – isoamyl alcohol mixture for precise extraction of microRNAs from biological fluids, contributing to cancer diagnostics and molecular pathology (Pero-Gascon et al., 2018 ).

The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs: Highlights the integration of the Phenol – chloroform – isoamyl alcohol mixture with novel technologies for forensic applications, enhancing DNA extraction processes critical in legal and criminal investigations (Nori and McCord, 2015 ).

Frequency of Epstein-Barr virus DNA sequences in human gliomas: Utilizes the Phenol – chloroform – isoamyl alcohol mixture for DNA extraction in medical research, facilitating the study of viral presence in cancer tissues and its implications for oncology and virology (Fonseca et al., 2015 ).

The critical role of DNA extraction for detection of mycobacteria in tissues: This study underscores the effectiveness of the Phenol – chloroform – isoamyl alcohol mixture in the molecular detection of pathogens, serving as a fundamental technique in microbiological diagnostics and infectious disease research (Radomski et al., 2013 ).

Physical form:

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 125:24:1 (v/v/v); saturated with 2 M NaOAc pH 4

Symbol	GHS05,GHS06,GHS08,GHS09
Signal word	Danger
Hazard statements	H301 + H311 + H331 - H314 - H341 - H351 - H361d - H372 - H373 - H411
Precautionary statements	P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
Hazard Codes	T
Risk Statements	63-23/24/25-34-40-48/20/21/22-68
Safety Statements	26-36/37/39-45
RIDADR	UN2810 - class 6.1 - PG 2 - EHS - Toxic, liquids, organic, n.o.s
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Density	1.12 g/mL at 20 °C
Storage Temp.	2-8°C
UNSPSC	12000000

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