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Propidium iodide

SIAL/81845 - ≥94% (HPLC)

Synonym: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

CAS Number: 25535-16-4
Empirical Formula (Hill Notation): C27H34I2N4
Molecular Weight: 668.39
EC Number: 247-081-0
MDL Number: MFCD00011921
Linear Formula: C27H34I2N4
Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-81845-25MG 25 mg
 $80.70
1/EA		 Add To Favorites
45-81845-100MG 100 mg
 $242.00
1/EA		 Add To Favorites

 

assay ≥94% (HPLC)
fluorescence λex 482 nm; λem 608 nm (pH 7.2)
  λex 540 nm; λem 608 nm (bound to DNA)
form powder
InChI 1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key XJMOSONTPMZWPB-UHFFFAOYSA-M
mp 220-225 °C (dec.) (lit.)
Quality Level 200 
SMILES string [I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
storage temp. 2-8°C
Application: in scrape-loading dye transfer assay
Application: Propidium iodide has been used:
• to stain DNA in cell cycle analysis
• to stain cells and cell nuclei
• for the preparation of propidium iodide (PI)stock solution
• to stain cells for flow cytometry
Features and Benefits: This compound is a featured product for Apoptosis research. Click here  to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm .
General description: PI is used for selective labelling of dead cells.
General description: Propidium iodide is a fluorescent intercalating agent used for staining. It functions as an apoptosis marker and has linear DNA-binding capacity. Propidium iodide is an effective indicator of tissue injury.
Packaging: 25, 100 mg in glass bottle
Packaging: Bottomless glass bottle. Contents are inside inserted fused cone.
Purity ≥94% (HPLC)
mp 220-225 °C (dec.) (lit.)
Storage Temp. 2-8°C
UNSPSC 12352200

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