Propidium iodide

SIAL/81845 - ≥94% (HPLC)

Synonym: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

CAS Number: 25535-16-4
Empirical Formula (Hill Notation): C₂₇H₃₄I₂N₄
Molecular Weight: 668.39
EC Number: 247-081-0
MDL Number: MFCD00011921
Linear Formula: C₂₇H₃₄I₂N₄
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-81845-25MG	25 mg	$89.00 1/EA	Add To Favorites
45-81845-100MG	100 mg	$267.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥94% (HPLC)
fluorescence	λ_ex 482 nm; λ_em 608 nm (pH 7.2)
	λ_ex 540 nm; λ_em 608 nm (bound to DNA)
form	powder
InChI	1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChI key	XJMOSONTPMZWPB-UHFFFAOYSA-M
mp	220-225 °C (dec.) (lit.)
Quality Level	200
SMILES string	[I-].[I-].CC[N+](C)(CC)CCC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14
storage temp.	2-8°C

Application:	in scrape-loading dye transfer assay
Application:	Propidium iodide has been used: • to stain DNA in cell cycle analysis • to stain cells and cell nuclei • for the preparation of propidium iodide (PI)stock solution • to stain cells for flow cytometry
Features and Benefits:	This compound is a featured product for Apoptosis research. Click here to discover more featured Apoptosis products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm .
General description:	PI is used for selective labelling of dead cells.
General description:	Propidium iodide is a fluorescent intercalating agent used for staining. It functions as an apoptosis marker and has linear DNA-binding capacity. Propidium iodide is an effective indicator of tissue injury.
Packaging:	25, 100 mg in glass bottle
Packaging:	Bottomless glass bottle. Contents are inside inserted fused cone.

Symbol	GHS08
Signal word	Warning
Hazard statements	H341
Precautionary statements	P201 - P202 - P280 - P308 + P313 - P405 - P501
Hazard Codes	Xn
Risk Statements	36/37/38-68
Safety Statements	26-36/37
RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥94% (HPLC)
mp	220-225 °C (dec.) (lit.)
Storage Temp.	2-8°C
UNSPSC	12352200

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